LBP Polyklonaler Antikörper

LBP Polyklonal Antikörper für FC, IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC,ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 11836-1-AP

Synonyme

LBP

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Galerie der Validierungsdaten

• 

  WB analysis of mouse liver using 11836-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11836-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11836-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 11836-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11836-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11836-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11836-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11836-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 11836-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 11836-1-AP

  IP Result of anti-LBP (IP:11836-1-AP, 4ug; Detection:11836-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human liver using 11836-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 11836-1-AP (LBP Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 11836-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 11836-1-AP (LBP Antibody) at dilution of 1:50.

• 

  IF Staining of L02 using 11836-1-AP

  Immunofluorescent analysis of L02 cells using 11836-1-AP (LBP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 11836-1-AP

  Immunofluorescent analysis of HepG2 cells, using LBP antibody 11836-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  FC experiment of HeLa using 11836-1-AP

  1X10^6 HeLa cells were stained with 0.2ug LBP antibody (11836-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, A431-Zellen, A549-Zellen, HeLa-Zellen, Jurkat-Zellen, L02-Zellen, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: L02-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

11836-1-AP bindet in WB, IP, IHC, IF, FC,ELISA LBP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LBP fusion protein Ag2431
• Vollständiger Name: lipopolysaccharide binding protein
• Berechnetes Molekulargewicht: 481 aa, 53 kDa
• Beobachtetes Molekulargewicht: 63-67 kDa
• GenBank-Zugangsnummer: BC022256
• Gene symbol: LBP
• Gene ID (NCBI): 3929
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LBP belongs to the BPI/LBP/Plunc superfamily and BPI/LBP family. It binds to the lipid A moiety of bacterial lipopolysaccharides (LPS) which is a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. LBP is involved in the acute-phase immunologic response to gram-negative bacterial infections. In many tissue/cells, we get 53-60kd and 63-67kd(Glycosylation form).

Publikationen

Species	Application	Title
		J Neurosurg; Association of decreased levels of lipopolysaccharide-binding protein with OKN-007-induced regression of tumor growth in an F98 rat glioma model.; Authors - Nataliya Smith
human	WB	Cancer Sci; Circulating exosomes contain protein biomarkers of metastatic non-small-cell lung cancer.; Authors - Ning Wang
rat	IHC	J Biomed Mater Res A; The characteristic regulation of gene expression Lbp and Sod3 in peri-implant connective tissue of rats.; Authors - Takafumi Kobayashi