LC3B Rekombinanter Antikörper

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• KD/KO Validated

LC3B Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(31)

CloneNo.

5P12

Kat-Nr. : 81004-1-RR

Synonyme

LC3, MAP1LC3B, 5P12, ATG8F, Autophagy-related ubiquitin-like modifier LC3 B

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  WB analysis using 81004-1-RR

  Untreated and chloroquine treated HEK-293 cells, untreated and chloroquine treated HeLa cells were subjected to SDS PAGE followed by western blot with 81004-1-RR (LC3B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 81004-1-RR

  WB result of LC3B antibody (81004-1-RR; 1:20000; room temperature for 1.5 hours) with negative Control and LC3 knockout HeLa cells.

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  WB analysis of mouse brain using 81004-1-RR

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 81004-1-RR (LC3B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 81004-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81004-1-RR (LC3B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 81004-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81004-1-RR (LC3B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 81004-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81004-1-RR (LC3B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 81004-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81004-1-RR (LC3B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 81004-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed Chloroquine treated HeLa cells using LC3B antibody (81004-1-RR, Clone: 5P12 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

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  Affinity and Kinetic Characterization of 81004-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81004-1-RR against Human LC3 were performed. The affinity constant is 0.967 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, mit Chloroquin behandelte HEK-293-Zellen, mit Chloroquin behandelte HeLa-Zellen, HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: mit Chloroquin behandelte HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 27 publications below
• IHC: See 1 publications below
• IF: See 11 publications below

Produktinformation

81004-1-RR bindet in WB, IHC, IF/ICC, ELISA LC3B und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: LC3B fusion protein Ag6144
• Vollständiger Name: microtubule-associated protein 1 light chain 3 beta
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 14-18 kDa
• GenBank-Zugangsnummer: BC067797
• Gene symbol: LC3B
• Gene ID (NCBI): 81631
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane. The antibody 81004-1-RR specifically recognizes LC3B.

Publikationen

Species	Application	Title
mouse	WB,IF	Cell Metab; Disrupted methionine cycle triggers muscle atrophy in cancer cachexia through epigenetic regulation of REDD1; Authors - Kai Lin
mouse	WB,IF	Int Immunopharmacol; N-acetylserotonin derivative ameliorates hypoxic-ischemic brain damage by promoting PINK1/Parkin-dependent mitophagy to inhibit NLRP3 inflammasome-induced pyroptosis; Authors - Fang Fang
bovine	WB	J Dairy Sci; Subacute ruminal acidosis induces pyroptosis via the mitophagy-mediated NLRP3 inflammasome activation in the livers of dairy cows fed a high-grain diet; Authors - Hongzhu Zhang
sheep	WB	J Dairy Sci; Short-term effects of Subacute ruminal acidosis on ferroptosis and iron metabolism in the livers of lactating sheep fed a high-grain diet; Authors - Hongzhu Zhang
human	WB	Discov Oncol; L-cysteine contributes to destructive activities of odontogenic cysts/tumor; Authors - Ji Li
human	WB	Cancers (Basel); Interfering Nuclear Protein Laminb1 Induces DNA Damage and Reduces Vemurafenib Resistance in Melanoma Cells In Vitro; Authors - Yuan Li