LCE1B Polyklonaler Antikörper

LCE1B Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21771-1-AP

Synonyme

LEP2, SPRL2A, Small proline-rich-like epidermal differentiation complex protein 2A, Late envelope protein 2, late cornified envelope 1B

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  WB analysis of A375 using 21771-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 21771-1-AP (LCE1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 21771-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 21771-1-AP (LCE1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skin using 21771-1-AP

  rat skin tissue were subjected to SDS PAGE followed by western blot with 21771-1-AP (LCE1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 21771-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 21771-1-AP (LCE1B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 21771-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 21771-1-AP (LCE1B antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human oesophagus using 21771-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 21771-1-AP (LCE1B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human oesophagus using 21771-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 21771-1-AP (LCE1B antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A375 using 21771-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using LCE1B antibody (21771-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, Maushautgewebe, Rattenhautgewebe
• Erfolgreiche Detektion in IHC: humanes Hautgewebe, humanes Ösophagusgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A375-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

21771-1-AP bindet in WB, IHC, IF/ICC, ELISA LCE1B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LCE1B fusion protein Ag16442
• Vollständiger Name: late cornified envelope 1B
• Berechnetes Molekulargewicht: 118 aa, 12 kDa
• Beobachtetes Molekulargewicht: 10-12 kDa
• GenBank-Zugangsnummer: BC104232
• Gene symbol: LCE1B
• Gene ID (NCBI): 353132
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human LCE1B (Late cornified envelope protein 1B), also named late envelope protein 2 (LEP2), is located at human Chromosome1q21 which contains multiple LCE clusters. LCE1B gene is predicated to encode a 118-aa protein, and is currently evidenced at transcript level. Its expression was skin-specific and was readily detected in adult trunk skin, adult arm skin, fetal skin, penal skin, vulva, esophagus and tongue (PMID: 15854049). LCE genes respond to environmental stimuli such as calcium levels and ultraviolet (UV) light, however, LCE groups have distinct functions (PMID: 15854049).