Lipocalin-2/NGAL Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Lipocalin-2/NGAL Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

25

Kat-Nr. : 26991-1-AP

Synonyme

24p3, HNL, LCN2, lipocalin 2, NGAL, Oncogene 24p3, p25

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Galerie der Validierungsdaten

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  WB analysis of human saliva using 26991-1-AP

  human saliva were subjected to SDS PAGE followed by western blot with 26991-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26991-1-AP

  HT-29 cells were subjected to SDS PAGE followed by western blot with 26991-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 26991-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26991-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 26991-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26991-1-AP (LCN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 26991-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26991-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 26991-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26991-1-AP (LCN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 26991-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using Lipocalin-2/NGAL antibody (26991-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of RAW 264.7 using 26991-1-AP

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using Lipocalin-2/NGAL antibody (26991-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  FC experiment of A431 using 26991-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Lipocalin-2/NGAL (26991-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, human saliva
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: RAW 264.7-Zellen, A431-Zellen
• Erfolgreiche Detektion in FC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 16 publications below
• IHC: See 8 publications below
• IF: See 5 publications below

Produktinformation

26991-1-AP bindet in WB, IHC, IF, FC, ELISA Lipocalin-2/NGAL und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Lipocalin-2/NGAL fusion protein Ag25715
• Vollständiger Name: lipocalin 2
• Beobachtetes Molekulargewicht: 25 kDa
• Gene symbol: LCN2
• Gene ID (NCBI): 3934
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lipocalin-2 (LCN-2) is a adipocytokine also referred to as neutrophil gelatinase-associated lipocalin (NGAL). Lipocalin-2 is a circulatory protein responsible for the transportation of small and hydrophobic molecules to target organs. Lipocalin-2 is used as a biomarker for acute and chronic renal injury. It is present in a large variety of cells including neutrophil, hepatocytes, lung, bone marrow, adipose tissue, macrophages, thymus, non-neoplastic breast duct, prostate, and renal cells. Different functions have been associated with Lipocalin-2, including antibacterial, anti-inflammatory, and protection against cell and tissue stress (PMID:34463264).

Publikationen

Species	Application	Title
human	WB	J Neurol Neurosurg Psychiatry; Molecules of senescent glial cells differentiate Alzheimer's disease from ageing; Authors - Linbin Dai
	WB	Cell Chem Biol; 3D two-photon brain imaging reveals dihydroartemisinin exerts antiepileptic effects by modulating iron homeostasis.; Authors - Chenwen Shao
human	WB	Oxid Med Cell Longev; Sirtuin 1 Induces Choroidal Neovascularization and Triggers Age-Related Macular Degeneration by Promoting LCN2 through SOX9 Deacetylation.; Authors - Su Zhao ; KD Validated
human	WB,IHC	Gastric Cancer; Lipocalin-2 negatively regulates epithelial-mesenchymal transition through matrix metalloprotease-2 downregulation in gastric cancer.; Authors - Sadaaki Nishimura ; KD Validated
human	IF	iScience; Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1; Authors - Lingxiao Tong
human	WB	iScience; IRP1 mediated ferroptosis reverses temozolomide resistance in glioblastoma via affecting LCN2/FPN1 signaling axis depended on NFKB2; Authors - Yufei Lan