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  • KD/KO Validated

Lipocalin-2/NGAL Polyklonaler Antikörper

Lipocalin-2/NGAL Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(46)

Kat-Nr. : 26991-1-AP

Synonyme

LCN2, lipocalin 2, NGAL, 24p3, 25 kDa alpha-2-microglobulin-related subunit of MMP-9



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHT-29-Zellen, human saliva
Erfolgreiche Detektion in IHChumanes Kolonkarzinomgewebe, humanes Magenkrebsgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCA431-Zellen
Erfolgreiche Detektion in FC (Intra)A431-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

26991-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA Lipocalin-2/NGAL und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Lipocalin-2/NGAL fusion protein Ag25715
Vollständiger Name lipocalin 2
Beobachtetes Molekulargewicht 25 kDa
Gene symbol NGAL/LCN2
Gene ID (NCBI) 3934
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lipocalin-2 (LCN-2) is a adipocytokine also referred to as neutrophil gelatinase-associated lipocalin (NGAL). Lipocalin-2 is a circulatory protein responsible for the transportation of small and hydrophobic molecules to target organs. Lipocalin-2 is used as a biomarker for acute and chronic renal injury. It is present in a large variety of cells including neutrophil, hepatocytes, lung, bone marrow, adipose tissue, macrophages, thymus, non-neoplastic breast duct, prostate, and renal cells. Different functions have been associated with Lipocalin-2, including antibacterial, anti-inflammatory, and protection against cell and tissue stress (PMID:34463264).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Lipocalin-2/NGAL antibody 26991-1-APProtokoll herunterladen
IHC protocol for Lipocalin-2/NGAL antibody 26991-1-APProtokoll herunterladenl
IF protocol for Lipocalin-2/NGAL antibody 26991-1-APProtokoll herunterladen
FC protocol for Lipocalin-2/NGAL antibody 26991-1-APDownload protocol
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

J Neurol Neurosurg Psychiatry

Molecules of senescent glial cells differentiate Alzheimer's disease from ageing

Authors - Linbin Dai
WB

Cell Chem Biol

3D two-photon brain imaging reveals dihydroartemisinin exerts antiepileptic effects by modulating iron homeostasis.

Authors - Chenwen Shao
humanWB

Oxid Med Cell Longev

Sirtuin 1 Induces Choroidal Neovascularization and Triggers Age-Related Macular Degeneration by Promoting LCN2 through SOX9 Deacetylation.

Authors - Su Zhao
  • KD Validated
humanWB,IHC

Gastric Cancer

Lipocalin-2 negatively regulates epithelial-mesenchymal transition through matrix metalloprotease-2 downregulation in gastric cancer.

Authors - Sadaaki Nishimura
  • KD Validated
humanWB,IHC

Biochim Biophys Acta Mol Basis Dis

Lipocalin-2-mediated intestinal epithelial cells pyroptosis via NF-κB/NLRP3/GSDMD signaling axis adversely affects inflammation in colitis

Authors - Yuyi Yang
humanIF

iScience

Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1

Authors - Lingxiao Tong