L-Plastin Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

L-Plastin Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 13025-1-AP

Synonyme

L PLASTIN, LCP 1, LCP1, L-Plastin, Lymphocyte cytosolic protein 1, Plastin 2, PLS2

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 13025-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13025-1-AP (L-Plastin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 13025-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13025-1-AP (L-Plastin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 13025-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13025-1-AP (L-Plastin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 13025-1-AP

  IP Result of anti-L-Plastin (IP:13025-1-AP, 4ug; Detection:13025-1-AP 1:1000) with Jurkat cells lysate 2400ug.

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  IHC staining of human lymphoma using 13025-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 13025-1-AP (L-Plastin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 13025-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13025-1-AP (L-Plastin antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 13025-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13025-1-AP (L-Plastin antibody at dilution of 1:50 (under 40x lens).

• 

  FC experiment of Raji using 13025-1-AP

  1X10^6 Raji cells were stained with 0.2ug L-Plastin antibody (13025-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, K-562-Zellen, Mausmilzgewebe
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• FC: See 1 publications below

Produktinformation

13025-1-AP bindet in WB, IP, IHC, FC, ELISA L-Plastin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, hamster, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: L-Plastin fusion protein Ag3571
• Vollständiger Name: lymphocyte cytosolic protein 1 (L-plastin)
• Berechnetes Molekulargewicht: 627 aa, 64 kDa
• Beobachtetes Molekulargewicht: 64-70 kDa
• GenBank-Zugangsnummer: BC010271
• Gene symbol: LCP1
• Gene ID (NCBI): 3936
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Sci Signal; A hepatokine derived from the ER protein CREBH promotes triglyceride metabolism by stimulating lipoprotein lipase activity; Authors - Hyunbae Kim
human	WB,IHC	Mol Ther Nucleic Acids; Exosomal Transfer of LCP1 Promotes Osteosarcoma Cell Tumorigenesis and Metastasis by Activating the JAK2/STAT3 Signaling Pathway.; Authors - Xuhui Ge
human	IHC	Cancer Biomark; LCP1 is a prognostic biomarker correlated with immune infiltrates in gastric cancer; Authors - Qingwen Zeng
mouse	WB	Drug Dev Res; Therapeutic role of Artemether in the prevention of hepatic steatosis through miR-34a-5p/PPARα pathway; Authors - Li Chen
monkey	IHC	Mol Biosyst; Unravelling the proteome of adult rhesus monkey ovaries.; Authors - Hui He
human	WB,FC	Pharm Res; Transcriptome Profiling Analysis Identifies LCP1 as a Contributor for Chidamide Resistance in Gastric Cancer.; Authors - Wenfang Bao ; KD Validated