LDHA-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

LDHA-Specific Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, RIP, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

181

Rezensionen (1)

Kat-Nr. : 19987-1-AP

Synonyme

lactate dehydrogenase A, LDH A, LDH M, LDH muscle subunit, LDH1, LDHA, LDHA-Specific, PIG19

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Galerie der Validierungsdaten

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  WB analysis using 19987-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19987-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19987-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19987-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19987-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 19987-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 19987-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19987-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19987-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 19987-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 19987-1-AP

  IP Result of anti-LDHA-Specific (IP:19987-1-AP, 4ug; Detection:19987-1-AP 1:2000) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of human heart using 19987-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 19987-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 19987-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 19987-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 19987-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 19987-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 19987-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 19987-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 19987-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using LDHA-Specific antibody (19987-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 19987-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using LDHA-Specific antibody (19987-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 19987-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug LDHA-Specific antibody (19987-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A431-Zellen, HeLa-Zellen, Jurkat-Zellen, Maushodengewebe, MCF-7-Zellen, NIH/3T3-Zellen, Rattennierengewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Hodengewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 8 publications below
• WB: See 158 publications below
• IHC: See 30 publications below
• IF: See 8 publications below
• IP: See 5 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

19987-1-AP bindet in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA LDHA-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: lactate dehydrogenase A
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 32-37 kDa
• GenBank-Zugangsnummer: NM_005566
• Gene symbol: LDHA
• Gene ID (NCBI): 3939
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lactate dehydrogenase (LDH) is composed of A subunits predominate in skeletal muscle and B subunits are abundantly produced in brain and heart. The LDHA (lactate dehydrogenase A) and COPB1 (coatomer protein complex, subunit beta 1)genes, are involved in energy metabolism and protein transport processes. Both genes might play important roles in muscle development. It has some isoforms with the molecular mass of 27-40 kDa and can form a homotetramer(PMID:11276087). This antibody is specific to LDHA and has no cross reaction to LDHB and LDHC.

Publikationen

Species	Application	Title
mouse	WB	Nat Cell Biol; RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis; Authors -
human	WB	Cancer Cell; Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.; Authors - Ling Li
human	WB	Drug Resist Updat; Targeting ACYP1-mediated glycolysis reverses lenvatinib resistance and restricts hepatocellular carcinoma progression; Authors - Shuai Wang ; KD Validated
human		Nat Immunol; NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.; Authors - Di Huang
human	WB	Cell Metab; Di-methylation of CD147-K234 Promotes the Progression of NSCLC by Enhancing Lactate Export.; Authors - Ke Wang
mouse	WB	ACS Nano; Dual Inhibition of Endoplasmic Reticulum Stress and Oxidation Stress Manipulates the Polarization of Macrophages under Hypoxia to Sensitize Immunotherapy.; Authors - Mengshi Jiang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Christian (Verified Customer) (01-07-2022)

We expected a huge expression in our primary human chondrocytes and it was perfectly and specifically confirmed by this antibody. The antibody worked also for primary rat chondrocytes. (cell lysis with RIPA-lysis buffer, denaturating conditions)

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: human primary chondrocytes

LDHA-Specific Antibody Western Blot validation (1:2000 dilution) in human primary chondrocytes (Cat no:19987-1-AP)

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