LDHB-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

LDHB-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 19988-1-AP

Synonyme

lactate dehydrogenase B, LDHB, EC:1.1.1.27, LDHA, LDH H

---

• 

  WB analysis using 19988-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19988-1-AP (LDHB-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19988-1-AP

  WB result of LDHB-Specific antibody (19988-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-LDHB-Specific transfected HeLa cells.

• 

  IHC staining of human ovary cancer using 19988-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19988-1-AP (LDHB-Specific antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 19988-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LDHB-Specific antibody (19988-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, HEK-293T-Zellen, HeLa-Zellen, Jurkat-Zellen, Mausherzgewebe, PC-3-Zellen, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:300-1:1200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

19988-1-AP bindet in WB, IHC, IF/ICC, ELISA LDHB-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: lactate dehydrogenase B
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: NM_002300
• Gene symbol: LDHB
• Gene ID (NCBI): 3945
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LDHB, also named as NY-REN-46 and LDH-H, belongs to the LDH/MDH superfamily and LDH family. LDHB catalyzes the reaction: (S)-lactate + NAD+ = pyruvate + NADH. It is an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway. It is glycolytic enzymes and it is positively regulated by mTOR.Defects in LDHB are a cause of hereditary LDHB deficiency. The antibody is specific to LDHB and has no cross reaction with LDHA and LDHC.

Publikationen

Species	Application	Title
mouse	WB	Biomedicines; HMTM-Mediated Enhancement of Brain Bioenergetics in a Mouse Tauopathy Model Is Blocked by Chronic Administration of Rivastigmine.; Authors - Renato X Santos
mouse	WB	J Nutr Sci Vitaminol (Tokyo); Combination of Exercise and Intake of Amino Acid Mixture Synergistically Induces Beige Adipocyte Formation in Mice; Authors - Takuya Kojima
human	WB	Mol Biol Rep; Development of a one-plasmid system to replace the endogenous protein with point mutation for post-translational modification studies.; Authors - Iqra Ishrat
mouse	IHC, IF	Am J Physiol Renal Physiol; Expression of lactate dehydrogenase A and B isoforms in the mouse kidney.; Authors - Gunars Osis
human	WB,IF	iScience; Lactate-driven type I collagen deposition facilitates cancer stem cell-like phenotype of head and neck squamous cell carcinoma; Authors - Yunqing Sun ; KD Validated
human	WB	Prostate; The Diagnostic Value of Plasma Small Extracellular Vesicle-Derived CAIX Protein in Prostate Cancer and Clinically Significant Prostate Cancer: A Study on Predictive Models; Authors - Haotian Chen