LDLR Monoklonaler Antikörper

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• KD/KO Validated

LDLR Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

CloneNo.

1A2A3

Kat-Nr. : 66414-1-Ig

Synonyme

1A2A3, FH, FHC, LDL receptor

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  WB analysis of HeLa using 66414-1-Ig

  WB result of LDLR antibody (66414-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LDLR transfected HeLa cells.

• 

  WB analysis using 66414-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66414-1-Ig (LDLR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66414-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66414-1-Ig (LDLR Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66414-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66414-1-Ig (LDLR Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66414-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66414-1-Ig (LDLR Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 66414-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66414-1-Ig (LDLR Antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human liver using 66414-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66414-1-Ig (LDLR Antibody) at dilution of 1:400 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, MCF-7-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:15000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below

Produktinformation

66414-1-Ig bindet in WB, IHC, ELISA LDLR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, hamster
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LDLR fusion protein Ag1236
• Vollständiger Name: low density lipoprotein receptor
• Berechnetes Molekulargewicht: 95 kDa
• Beobachtetes Molekulargewicht: 100-160 kDa
• GenBank-Zugangsnummer: BC014514
• Gene symbol: LDLR
• Gene ID (NCBI): 3949
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LDLR (low density lipoprotein receptor) is a member of the LDL receptor gene family and is involved in receptor-mediated endocytosis of specific ligands. The LDLR is a cell surface glycoprotein that scavenges LDL from the blood and regulates plasma LDL cholesterol. The cytoplasmic domain of the LDL receptor is necessary for the receptor to cluster in coated pits, which promotes the rapid endocytosis of bound LDL. The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 to 160 kDa bands on SDS-PAGE.

Publikationen

Species	Application	Title
human	WB	Acta Pharmacol Sin; AMPK activator C24 inhibits hepatic lipogenesis and ameliorates dyslipidemia in HFHC diet-induced animal models.; Authors - Shui-Mei Sun
	WB	Skelet Muscle; Limb-girdle muscular dystrophy type 2B causes HDL-C abnormalities in patients and statin-resistant muscle wasting in dysferlin-deficient mice; Authors - Zoe White
human	WB	Research (Wash D C); Large-Scale CRISPR Screen of LDLR Pathogenic Variants; Authors - Mengjing Li
human	WB	Nat Nanotechnol; Nanoparticles for inducing Gaucher disease-like damage in cancer cells; Authors - Chunyan Yue ; KD Validated
human,hamster	WB	Int J Mol Sci; Oncolytic Activity of Sindbis Virus with the Help of GM-CSF in Hepatocellular Carcinoma; Authors - Xiangwei Shi
		Biochem Biophys Res Commun; Low-density Lipoprotein Receptor is an important host factor in flaviviral entry and replication in neurons; Authors - Meenakshi Bhaskar