LENG9 Polyklonaler Antikörper

LENG9 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 16295-1-AP

Synonyme

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  WB analysis of HeLa using 16295-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16295-1-AP (LENG9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 16295-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 16295-1-AP (LENG9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 16295-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LENG9 antibody (16295-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of MCF-7 using 16295-1-AP

  Immunofluorescent analysis of MCF-7 cells, using LENG9 antibody 16295-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Y79-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Hirngewebe, humanes Herzgewebe, humanes Lebergewebe, humanes Lungengewebe, humanes Plazenta-Gewebe, humanes Hautgewebe, humanes Milzgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

16295-1-AP bindet in WB, IHC, IF/ICC, ELISA LENG9 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LENG9 fusion protein Ag9481
• Vollständiger Name: leukocyte receptor cluster (LRC) member 9
• Berechnetes Molekulargewicht: 501 aa, 53 kDa
• Beobachtetes Molekulargewicht: 54-60 kDa
• GenBank-Zugangsnummer: BC015921
• Gene symbol: LENG9
• Gene ID (NCBI): 94059
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LENG9 is rarely reported in the literature. GO function analysis revealed that the molecular function of LENG9 is metal ion binding (GO:0046872), and the BioGRID database showed that LENG9 has 6 interactors, including C9ORF41, B4GALT2, CDC5L, D2HGDH, FOXS1 and OGT. Studies have shown that LENG9 may play an oncogenic role in tumor pathogenesis by promoting tumor cell proliferation, migration, and invasion. (PMID: 35538075)

Publikationen

Species	Application	Title
human	WB	Nat Commun; Long-read sequencing unveils high-resolution HPV integration and its oncogenic progression in cervical cancer.; Authors - Liyuan Zhou