Leptin Polyklonaler Antikörper

Leptin Polyklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 17436-1-AP

Synonyme

LEP, leptin, OB, Obese protein, Obesity factor, OBS

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Galerie der Validierungsdaten

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  IHC staining of human stomach using 17436-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17436-1-AP (Leptin antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human stomach using 17436-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17436-1-AP (Leptin antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of mouse ovary using 17436-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 17436-1-AP (Leptin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 17436-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 17436-1-AP (Leptin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17436-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17436-1-AP (Leptin antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 17436-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17436-1-AP (Leptin antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human ovary using 17436-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 17436-1-AP (Leptin antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human ovary using 17436-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 17436-1-AP (Leptin antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human placenta using 17436-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17436-1-AP (Leptin antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human placenta using 17436-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17436-1-AP (Leptin antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 17436-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17436-1-AP (Leptin antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 17436-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17436-1-AP (Leptin antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 17436-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 17436-1-AP (Leptin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 17436-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 17436-1-AP (Leptin antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 17436-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17436-1-AP (Leptin antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 17436-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17436-1-AP (Leptin antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 17436-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17436-1-AP (Leptin antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 17436-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17436-1-AP (Leptin antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse brain using 17436-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17436-1-AP (Leptin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17436-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17436-1-AP (Leptin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 17436-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 17436-1-AP (Leptin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of NIH/3T3 using 17436-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 17436-1-AP (Leptin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Magengewebe, humanes Eierstockgewebe, humanes Hodengewebe, humanes Lebergewebe, humanes Mammakarzinomgewebe, humanes Plazenta-Gewebe, Maus-Eierstockgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 3 publications below

Produktinformation

17436-1-AP bindet in IHC, IF, ELISA Leptin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Leptin fusion protein Ag11507
• Vollständiger Name: leptin
• Berechnetes Molekulargewicht: 167 aa, 19 kDa
• GenBank-Zugangsnummer: BC060830
• Gene symbol: LEP
• Gene ID (NCBI): 3952
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Leptin is a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. Leptin is the most critical hormone in the homeostatic regulation of energy balance among those so far discovered. Leptin primarily acts on the neurons of the mediobasal part of hypothalamus to regulate food intake, thermogenesis, and the blood glucose level. Leptin also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. Leptin has also been linked to type 2 diabetes mellitus development.

Publikationen

Species	Application	Title
human	IHC	Biomedicines; Leptin, Leptin Receptor, KHDRBS1 (KH RNA Binding Domain Containing, Signal Transduction Associated 1), and Adiponectin in Bone Metastasis from Breast Carcinoma: An Immunohistochemical Study.; Authors - Paola Maroni
mouse		Mol Immunol; Inflammatory arthritis increases the susceptibility to acute immune-mediated hepatitis in mice through enhancing leptin expression in T cells; Authors - Youyi Wang
mouse	IHC	Biomed Res Int; Polydatin Inhibits Adipose Tissue Inflammation and Ameliorates Lipid Metabolism in High-Fat-Fed Mice.; Authors - Li Zheng
mouse	IHC	J Immunol; Leptin Promotes Monosodium Urate Crystal-Induced Inflammation in Human and Murine Models of Gout.; Authors - Yiyun Yu