LETM1 Monoklonaler Antikörper

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• KD/KO Validated

LETM1 Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

2B9B9

Kat-Nr. : 68208-1-Ig

Synonyme

LETM1

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Galerie der Validierungsdaten

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  WB analysis using 68208-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 68208-1-Ig (LETM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of rat heart using 68208-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 68208-1-Ig (LETM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 68208-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LETM1 antibody (68208-1-Ig, Clone: 2B9B9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of U-251 using 68208-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using LETM1 antibody (68208-1-Ig, Clone: 2B9B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 68208-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LETM1 antibody (68208-1-Ig, Clone: 2B9B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, Rattenherzgewebe
• Erfolgreiche Detektion in IF: HeLa-Zellen, U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68208-1-Ig bindet in WB, IF, ELISA LETM1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LETM1 fusion protein Ag8851
• Vollständiger Name: leucine zipper-EF-hand containing transmembrane protein 1
• Berechnetes Molekulargewicht: 739 aa, 83 kDa
• Beobachtetes Molekulargewicht: 83 kDa
• GenBank-Zugangsnummer: BC021208
• Gene symbol: LETM1
• Gene ID (NCBI): 3954
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LETM1 is a mitochondrial inner membrane protein. It functions to maintain the mitochondrial tubular shapes and is required for normal mitochondrial morphology and cellular viability. It served as an anchor protein for complex formation between mitochondria and ribosome, and regulated mitochondrial biogenesis. It was also identified inWolf-Hirschhorn syndrome, and was deleted in nearly all patients with the syndrome. A recent study determined that adenovirus-mediated LETM1 may provide a useful target for designing lung tumor prevention and treatment.