LGR5 Polyklonaler Antikörper

LGR5 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 21833-1-AP

Synonyme

FEX, G protein coupled receptor 49, G protein coupled receptor 67, GPR49, GPR67, GRP49, HG38, LGR5

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  IHC staining of human small intestine using 21833-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 21833-1-AP (LGR5 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 21833-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 21833-1-AP (LGR5 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human small intestine using 21833-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 21833-1-AP (LGR5 antibody) at dilution of 1:800 (under 10x lens).

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  IHC staining of human small intestine using 21833-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 21833-1-AP (LGR5 antibody) at dilution of 1:800 (under 40x lens).

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  IHC staining of human colon cancer using 21833-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 21833-1-AP (LGR5 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 21833-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 21833-1-AP (LGR5 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human small intestine using 21833-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 21833-1-AP (LGR5 antibody) at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of human small intestine using 21833-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 21833-1-AP (LGR5 antibody) at dilution of 1:1000 (under 40x lens).

• 

  IHC staining of human liver cancer using 21833-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 21833-1-AP (LGR5 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 21833-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 21833-1-AP (LGR5 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 21833-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 21833-1-AP (LGR5 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 21833-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 21833-1-AP (LGR5 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Dünndarmgewebe, humanes Kolonkarzinomgewebe, humanes Kolongewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

21833-1-AP bindet in WB, IHC, IF, ELISA LGR5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LGR5 fusion protein Ag16389
• Vollständiger Name: leucine-rich repeat-containing G protein-coupled receptor 5
• Berechnetes Molekulargewicht: 907 aa, 100 kDa
• GenBank-Zugangsnummer: BC096324
• Gene symbol: LGR5
• Gene ID (NCBI): 8549
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LGR5, also named as GPR49, GPR67 and HG38, belongs to the G-protein coupled receptor 1 family. It is an orphan receptor. LGR5 is a stem cell marker of the intestinal epithelium and the hair follicule. It is a target gene of Wnt signaling.

Publikationen

Species	Application	Title
human	IHC	Oncotarget; LGR5 regulates pro-survival MEK/ERK and proliferative Wnt/β-catenin signalling in neuroblastoma.; Authors - Gabriella Cunha Vieira
human	IF	Inflamm Bowel Dis; Overexpression of Cancer-Associated Stem Cell Gene OLFM4 in the Colonic Epithelium of Patients With Primary Sclerosing Cholangitis.; Authors - Mastura Neyazi
mouse	WB	J Dermatol Sci; REGγ regulates hair cycle by activating Lgr5 positive hair follicle stem cells.; Authors - Xiao Gao
		Tumour Biol; Correlation of Musashi-1, Lgr5, and pEGFR expressions in human small intestinal adenocarcinomas.; Authors - Yan Wang
		Oncol Lett; Detection of circulating colorectal cancer cells by a custom microfluid system before and after endoscopic metallic stent placement.; Authors - Rei Ishibashi
human	IHC	Oncol Lett; Expression and clinical significance of SALL4 and LGR5 in patients with lung cancer.; Authors - Ajay Kumar Gautam