LIG4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

LIG4 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

22

Kat-Nr. : 12695-1-AP

Synonyme

DNA ligase 4, DNA ligase IV, LIG4, ligase IV, DNA, ATP dependent

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 12695-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12695-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12695-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12695-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 12695-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IHC staining of human prostate cancer using 12695-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12695-1-AP (LIG4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 12695-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 12695-1-AP (LIG4 Antibody) at dilution of 1:50.

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  IHC staining of human prostate cancer using 12695-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 12695-1-AP (LIG4 Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, HeLa-Zellen, HepG2-Zellen, Mauslebergewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 21 publications below
• IF: See 2 publications below

Produktinformation

12695-1-AP bindet in WB, IHC, IF, ELISA LIG4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LIG4 fusion protein Ag3385
• Vollständiger Name: ligase IV, DNA, ATP-dependent
• Berechnetes Molekulargewicht: 911 aa, 104 kDa
• Beobachtetes Molekulargewicht: 100-104 kDa
• GenBank-Zugangsnummer: BC037491
• Gene symbol: LIG4
• Gene ID (NCBI): 3981
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Two major pathways, homologous recombination (HR) and nonhomologous end joining (NHEJ), counteract one of themost toxic lesions, the DSB. The core protein complex mediating NHEJ in mammals includes DNA ligase IV (Lig4). Lig4 belongs to an ATP-dependent DNA ligase family, and joins single-strand brdownloadeaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. The complex Lig4-XRCC4 is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of Lig4.

Publikationen

Species	Application	Title
human	WB	Cell Host Microbe; Cellular DNA ligase I is recruited to cytoplasmic vaccinia virus factories and masks the role of the vaccinia ligase in viral DNA replication.; Authors - Paran Nir N ; KD Validated
human	WB	Nat Commun; Reciprocal regulation of RIG-I and XRCC4 connects DNA repair with RIG-I immune signaling.; Authors - Guijie Guo
human	IF	J Allergy Clin Immunol; XRCC4 deficiency in human subjects causes a marked neurological phenotype but no overt immunodeficiency.; Authors - Chaowan Guo
mouse	WB	Nat Commun; Rap1 regulates hematopoietic stem cell survival and affects oncogenesis and response to chemotherapy.; Authors - Ekta Khattar
human	WB	Nat Commun; Interactome analysis identifies a new paralogue of XRCC4 in non-homologous end joining DNA repair pathway.; Authors - Mengtan Xing
human	WB	Cancer Res; Inhibition of ATM Induces Hypersensitivity to Proton Irradiation by Upregulating Toxic End Joining.; Authors - Qin Zhou