EPLIN Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

EPLIN Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

6E6E6

Kat-Nr. : 66071-1-Ig

Synonyme

LIMA1, 6E6E6, LIM domain and actin binding 1, LIM domain and actin-binding protein 1, PP624

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  WB analysis of HEK-293 using 66071-1-Ig

  WB result of EPLIN antibody (66071-1-Ig, 1:2000) with si-Control and si-EPLIN transfected HEK293 cells.

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  WB analysis using 66071-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66071-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66071-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 66071-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66071-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 66071-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 66071-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 66071-1-Ig(EPLIN antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 66071-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 66071-1-Ig(EPLIN antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 66071-1-Ig

  Immunofluorescent analysis of Hela cells using 66071-1-Ig (EPLIN antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, COLO 320-Zellen, HaCaT-Zellen, HEK-293-Zellen, HT-29-Zellen, humanes Hirngewebe, MDA-MB-231-Zellen, Hausschwein-Kolongewebe, Ratten-Kolongewebe, ROS1728-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• CoIP: See 2 publications below

Produktinformation

66071-1-Ig bindet in WB, IHC, IF/ICC, CoIP, ELISA EPLIN und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EPLIN fusion protein Ag13288
• Vollständiger Name: LIM domain and actin binding 1
• Berechnetes Molekulargewicht: 85 kDa
• Beobachtetes Molekulargewicht: 90 kDa, 110 kDa
• GenBank-Zugangsnummer: BC001247
• Gene symbol: EPLIN
• Gene ID (NCBI): 51474
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EPLIN is a cytoskeletal protein that is preferentially expressed in epithelial cells and is implicated in regulation of cytoskeletal dynamics and cytokinesis. In human, there are two known isoforms, EPLIN-a and -b, generated by alternative promoter usage from a single gene. EPLIN-a expression is often down-regulated in cancerous cells and tissues. This antibody recognizes both of isoforms of EPLN.

Publikationen

Species	Application	Title
human	WB,IHC,IF	Adv Sci (Weinh); LIMA1 O-GlcNAcylation Promotes Hepatic Lipid Deposition through Inducing β-catenin-Regulated FASn Expression in Metabolic Dysfunction-Associated Steatotic Liver Disease; Authors - Fuji Yang
human	WB,CoIP	Cell Biol Toxicol; M6A modification-mediated LIMA1 promotes the progression of hepatocellular carcinoma through the wnt-βcatenin/Hippo pathway; Authors - Chao Zhang
mouse,human	WB,IHC,IF,CoIP	Cell Mol Biol Lett; Lipotoxic hepatocyte derived LIMA1 enriched small extracellular vesicles promote hepatic stellate cells activation via inhibiting mitophagy; Authors - Shihui Li ; KD Validated