LIN41 Polyklonaler Antikörper

LIN41 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 55003-1-AP

Synonyme

TRIM71, RING-type E3 ubiquitin transferase TRIM71, Protein lin-41 homolog, Protein lin 41 homolog, LIN 41

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• 

  WB analysis of mouse kidney using 55003-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55003-1-AP (LIN41 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 55003-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55003-1-AP (LIN41 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 55003-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 55003-1-AP (LIN41 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 55003-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55003-1-AP (LIN41 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 55003-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55003-1-AP (LIN41 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, DU 145-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

55003-1-AP bindet in WB, IHC, ELISA LIN41 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tripartite motif-containing 71
• Berechnetes Molekulargewicht: 93 kDa
• Beobachtetes Molekulargewicht: 93 kDa
• GenBank-Zugangsnummer: NM_001039111
• Gene symbol: LIN41
• Gene ID (NCBI): 131405
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRIM71, also named as LIN41, belongs to the TRIM/RBCC family. It may be involved in controlling the timing of organ formation during development. The antibody is specific to TRIM71.

Publikationen

Species	Application	Title
human	WB	Cell Cycle; TRIM71 binds to IMP1 and is capable of positive and negative regulation of target RNAs.; Authors - Daniel J Foster
human	WB	Cell Mol Gastroenterol Hepatol; HDLBP promotes hepatocellular carcinoma proliferation and sorafenib resistance by suppressing Trim71-dependent RAF1 degradation; Authors - Jingsheng Yuan
human,mouse	IHC	Mol Carcinog; Circ_0007429/miR-637/TRIM71/Ago2 axis participates in the regulation of proliferation, migration, invasion, apoptosis, and aerobic glycolysis of HCC; Authors - Lilan Fan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Daniel (Verified Customer) (10-26-2022)

This antibody produces very high background, using either milk or Licor blocking buffers, PVDF or nitrocellulose.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HeLa, MT4 cells

LIN41 Antibody Western Blot validation (1:500 dilution) in HeLa, MT4 cells (Cat no:55003-1-AP)