LIN7C Polyklonaler Antikörper

LIN7C Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Rezensionen (1)

Kat-Nr. : 14656-1-AP

Synonyme

VELI3, Protein lin-7 homolog C, Mammalian lin-seven protein 3, MALS-3, MALS3

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• 

  WB analysis of human cerebellum using 14656-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 14656-1-AP (LIN7C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 14656-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14656-1-AP (LIN7C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 14656-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14656-1-AP (LIN7C antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 14656-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14656-1-AP (LIN7C antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14656-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14656-1-AP (LIN7C antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Cerebellum-Gewebe, humanes Hirngewebe, humanes Nierengewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

14656-1-AP bindet in WB, IHC, IF/ICC, ELISA LIN7C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LIN7C fusion protein Ag6265
• Vollständiger Name: lin-7 homolog C (C. elegans)
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC053907
• Gene symbol: LIN7C
• Gene ID (NCBI): 55327
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	WB	Neuroscience; Proteome analysis of actin filament-associated proteins in the postnatal rat cerebellum.; Authors - Shi N N

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Thomas (Verified Customer) (11-17-2020)

Good specifity, also binds canine Lin7c

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HCT116 (human, colon cancer cell line), Caco2 (human, colorectal adenocarcinoma cell line) , MDCK (canine, kidney tubule)

LIN7C Antibody Western Blot validation (1:1000 dilution) in HCT116 (human, colon cancer cell line), Caco2 (human, colorectal adenocarcinoma cell line) , MDCK (canine, kidney tubule) (Cat no:14656-1-AP)