LMCD1 Monoklonaler Antikörper

LMCD1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

2A7B3

Kat-Nr. : 67834-1-Ig

Synonyme

Dyxin, LMCD1

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  WB analysis of U2OS using 67834-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 67834-1-Ig (LMCD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 67834-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67834-1-Ig (LMCD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 67834-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 67834-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat skeletal muscle using 67834-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skeletal muscle using 67834-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67834-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67834-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 67834-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 67834-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 67834-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using LMCD1 antibody (67834-1-Ig, Clone: 2A7B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U2OS-Zellen, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Kolonkarzinomgewebe, Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

67834-1-Ig bindet in WB, IHC, IF/ICC, ELISA LMCD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LMCD1 fusion protein Ag28331
• Vollständiger Name: LIM and cysteine-rich domains 1
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: ~40 kDa
• GenBank-Zugangsnummer: BC000646
• Gene symbol: LMCD1
• Gene ID (NCBI): 29995
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LIM and cysteine-rich domains-1 (LMCD1) is a member of the LIM protein family, which contains an N-terminal cysteine-rich region, two C-terminal LIM domains and a central PET (Prickle, Espinas, and Testin) domain. LMCD1 has been reported in cardiac tissues and lung acting as a transcriptional repressor for GATA6. The mutations of LMCD1 promote cell migration and tumor metastasis in hepatocellular carcinoma. (PMID: 31501411) It has calculated molecular weight around 41kDa.

Publikationen

Species	Application	Title
human,mouse	WB	Exp Eye Res; TGF-β1-mediated upregulation of LMCD1 drives corneal myofibroblast differentiation and corneal fibrosis; Authors - Yunlan Tang