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  • KD/KO Validated

LONP1 Polyklonaler Antikörper

LONP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(111)

Kat-Nr. : 15440-1-AP

Synonyme

PIM1, Mitochondrial ATP-dependent protease Lon, LONP 1, LONP, LON



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, humanes Herzgewebe, Jurkat-Zellen, Maushirngewebe, Mausherzgewebe, Rattenhirngewebe, Rattenherzgewebe, Rattenlungengewebe
Erfolgreiche IPMausherzgewebe
Erfolgreiche Detektion in IHChumanes Lungenkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:6000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

15440-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA LONP1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Affe, Huhn, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen LONP1 fusion protein Ag7196
Vollständiger Name lon peptidase 1, mitochondrial
Berechnetes Molekulargewicht 106 kDa
Beobachtetes Molekulargewicht 100 kDa
GenBank-ZugangsnummerBC000235
Gene symbol LONP1
Gene ID (NCBI) 9361
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then progressively degraded(PMID:15870080). LONP1 has some isoforms with the MW of 106 kDa, 100 kDa and 86 kDa.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for LONP1 antibody 15440-1-APProtokoll herunterladen
IHC protocol for LONP1 antibody 15440-1-APProtokoll herunterladenl
IF protocol for LONP1 antibody 15440-1-APProtokoll herunterladen
IP protocol for LONP1 antibody 15440-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Nature

A cytosolic surveillance mechanism activates the mitochondrial UPR

Authors - F X Reymond Sutandy
  • KD Validated
humanWB

Cell Metab

A High-Density Human Mitochondrial Proximity Interaction Network.

Authors - Hana Antonicka
monkeyWB,IF

Nat Cell Biol

MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.

Authors - Tim König
humanWB

Mol Cell

The mitochondrial DNAJC co-chaperone TCAIM reduces α-ketoglutarate dehydrogenase protein levels to regulate metabolism

Authors - Wang Jiahui
  • KD Validated
humanWB

Mol Cell

Circadian Oscillation of Sulfiredoxin in the Mitochondria.

Authors - In Sup Kil
  • KD Validated
mouseWB

Sci Adv

Fidelity of translation initiation is required for coordinated respiratory complex assembly.

Authors - Danielle L Rudler

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

B (Verified Customer) (01-28-2023)

It is okay to detect endogenous LONP1 although there are weak non specific bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse cortex lysate
LONP1 Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:15440-1-AP)