Loricrin Polyklonaler Antikörper

Loricrin Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(29)

Kat-Nr. : 55439-1-AP

Synonyme

LOR, LRN

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  IHC staining of mouse skin using 55439-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 55439-1-AP (Loricrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 55439-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 55439-1-AP (Loricrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 55439-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 55439-1-AP (Loricrin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 55439-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 55439-1-AP (Loricrin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human paracancerous tissue of skin using 55439-1-AP

  Immunohistochemical analysis of paraffin-embedded human paracancerous tissue of skin slide using 55439-1-AP (Loricrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human paracancerous tissue of skin using 55439-1-AP

  Immunohistochemical analysis of paraffin-embedded human paracancerous tissue of skin slide using 55439-1-AP (Loricrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 55439-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Loricrin antibody (55439-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maushautgewebe, human paracancerous tissue of skin; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 15 publications below
• IHC: See 10 publications below
• IF: See 11 publications below

Produktinformation

55439-1-AP bindet in WB, IHC, IF/ICC, ELISA Loricrin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: loricrin
• Berechnetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: NM_000427
• Gene symbol: Loricrin
• Gene ID (NCBI): 4014
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Loricrin, also named LOR and LRN, is a major component of the cornified cell envelope (CE) expressed late in epidermal differentiation in the granular layers of normal skin. This antibody is specific to Loricrin.

Publikationen

Species	Application	Title
mouse	WB,IF	Cell Death Differ; ENKD1 promotes epidermal stratification by regulating spindle orientation in basal keratinocytes.; Authors - Tao Zhong
mouse	WB	EMBO Rep; Regulation of epidermal differentiation through KDF1-mediated deubiquitination of IKKα.; Authors - Yuanyuan Li
mouse,human	WB,IF	Br J Pharmacol; FGF21 promotes migration and differentiation of epidermal cells during wound healing via SIRT1 dependent autophagy.; Authors - Xixi Chen
human	IF	J Nanobiotechnology; Exosomes from human induced pluripotent stem cells-derived keratinocytes accelerate burn wound healing through miR-762 mediated promotion of keratinocytes and endothelial cells migration.; Authors - Yunyao Bo
mouse	WB,IF	J Ethnopharmacol; Topical application of Artemisia annua L. essential oil ameliorates 2,4-dintrochlorobenzene-induced atopic dermatitis in mice; Authors - Zhiya Huang
human	WB	J Eur Acad Dermatol Venereol; Differentially expressed proteins identified by TMT proteomics analysis in children with verrucous epidermal naevi.; Authors - T Yuan