Loricrin Polyklonaler Antikörper

Loricrin Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

19

Kat-Nr. : 55439-1-AP

Synonyme

LOR, loricrin, LRN

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Galerie der Validierungsdaten

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  WB analysis of A431 using 55439-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55439-1-AP (Loricrin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin using 55439-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 55439-1-AP (Loricrin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 55439-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 55439-1-AP (Loricrin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin cancer using 55439-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 55439-1-AP (Loricrin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin cancer using 55439-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 55439-1-AP (Loricrin antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A431 using 55439-1-AP

  Immunofluorescent analysis of A431 cells using 55439-1-AP (Loricrin antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautgewebe, humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 12 publications below
• IHC: See 6 publications below
• IF: See 7 publications below

Produktinformation

55439-1-AP bindet in WB, IHC, IF, ELISA Loricrin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: loricrin
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: NM_000427
• Gene symbol: LOR
• Gene ID (NCBI): 4014
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Loricrin, also named LOR and LRN, is a major component of the cornified cell envelope (CE) expressed late in epidermal differentiation in the granular layers of normal skin. This antibody is specific to Loricrin.

Publikationen

Species	Application	Title
mouse	WB,IF	Cell Death Differ; ENKD1 promotes epidermal stratification by regulating spindle orientation in basal keratinocytes.; Authors - Tao Zhong
mouse	WB	EMBO Rep; Regulation of epidermal differentiation through KDF1-mediated deubiquitination of IKKα.; Authors - Yuanyuan Li
human,mouse	WB,IF	Br J Pharmacol; FGF21 promotes migration and differentiation of epidermal cells during wound healing via SIRT1 dependent autophagy.; Authors - Xixi Chen
human	IF	J Nanobiotechnology; Exosomes from human induced pluripotent stem cells-derived keratinocytes accelerate burn wound healing through miR-762 mediated promotion of keratinocytes and endothelial cells migration.; Authors - Yunyao Bo
human	WB	J Eur Acad Dermatol Venereol; Differentially expressed proteins identified by TMT proteomics analysis in children with verrucous epidermal naevi.; Authors - T Yuan
human,mouse	IHC,IF	Int J Biol Macromol; Oat β-glucan ameliorates epidermal barrier disruption by upregulating the expression of CaSR through dectin-1-mediated ERK and p38 signaling pathways.; Authors - Shuang Gao

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