LOXL2 Monoklonaler Antikörper

LOXL2 Monoklonal Antikörper für WB, IF, IHC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

CloneNo.

1H7F1

Rezensionen (1)

Kat-Nr. : 67139-1-Ig

Synonyme

LOR2, LOXL2, Lysyl oxidase homolog 2, lysyl oxidase like 2, Lysyl oxidase like protein 2, WS9 14

---

• 

  WB analysis of A549 using 67139-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 67139-1-Ig (LOXL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 67139-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67139-1-Ig (LOXL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 67139-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 67139-1-Ig (LOXL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 67139-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 67139-1-Ig (LOXL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat colon using 67139-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67139-1-Ig (LOXL2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 67139-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67139-1-Ig (LOXL2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 67139-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67139-1-Ig (LOXL2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, A375-Zellen, humanes Herzgewebe, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: Ratten-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:4000-1:16000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

67139-1-Ig bindet in WB, IHC, IF/ICC, ELISA LOXL2 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LOXL2 fusion protein Ag28566
• Vollständiger Name: lysyl oxidase-like 2
• Berechnetes Molekulargewicht: 774 aa, 87 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC000594
• Gene symbol: LOXL2
• Gene ID (NCBI): 4017
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LOXL2, also named as WS9-14, belongs to an amine oxidase family whose members have been implicated in crosslink formation in stromal collagens and elastin, cell motility, and tumor development and progression. LOXL2 activity might be a crucial modulator of Snail, providing an additional control mechanism of EMT and tumor progression. Increased LOXL2 expression in colon and esophageal cancer may contribute to tumor progression. We detected a band about 100 kd which is a secretory mature LOXL2 protein modified by glycosylation (PMID: 24014025).

Publikationen

Species	Application	Title
human		J Clin Invest; LOXL2-induced PEAR1 Ser891 phosphorylation suppresses CD44 degradation and promotes triple-negative breast cancer metastasis; Authors - Yingzhi Shen
mouse	WB	Aging Cell; Lysyl oxidase-like 2 inhibitor rescues D-galactose-induced skeletal muscle fibrosis.; Authors - Yongxin Wu
mouse	WB,IF	Int J Biol Macromol; Depletion of LOXL2 improves respiratory capacity: From air-breathing fish to mammal under hypoxia.; Authors - Bing Sun
human	WB	Oncogene; METTL1/WDR4-mediated tRNA m7G modification and mRNA translation control promote oncogenesis and doxorubicin resistance; Authors - Zhaoyu Wang
human	IF	Neuron; Single-cell dissection of the human blood-brain barrier and glioma blood-tumor barrier; Authors - Yuan Xie
human	WB,IHC	Neoplasia; β-Catenin mediated TAM phenotype promotes pancreatic cancer metastasis via the OSM/STAT3/LOXL2 axis; Authors - Yijia Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Boyan (Verified Customer) (10-04-2021)

The calculated MW is 87 kd. This antibody recognized major bands at 65, and 60 kd.

• Applications: Western Blot