LPHN3 Polyklonaler Antikörper

LPHN3 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 20045-1-AP

Synonyme

CIRL 3, CIRL3, KIAA0768, latrophilin 3, LEC3, Lectomedin 3, LPHN3

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  WB analysis of mouse brain using 20045-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 20045-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 20045-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 20045-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20045-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 20045-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 20045-1-AP

  IP result of anti-LPHN3 (IP:20045-1-AP, 4ug; Detection:20045-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, COLO 320-Zellen, HepG2-Zellen, humanes Lebergewebe, Maus-Kolongewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

20045-1-AP bindet in WB, IP, ELISA LPHN3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: latrophilin 3
• Berechnetes Molekulargewicht: 162 kDa
• Beobachtetes Molekulargewicht: 139 kDa
• GenBank-Zugangsnummer: NM_015236
• Gene symbol: LPHN3
• Gene ID (NCBI): 23284
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LPHN3, also named as KIAA0768 and LEC3, belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. LPHN3 forms a heterodimer, consisting of a large extracellular region (p120) non-covalently linked to a seven-transmembrane moiety (p85). This antibody recognizes all the isoforms of LPHN3 and has no cross reaction with LPHN1 and LPHN2.

Publikationen

Species	Application	Title
human	WB	World J Biol Psychiatry; Expression of the adult ADHD-associated gene ADGRL3 is dysregulated by risk variants and environmental risk factors.; Authors - Rhiannon V McNeill
human	WB	Nat Microbiol; Brain exposure to SARS-CoV-2 virions perturbs synaptic homeostasis; Authors - Emma Partiot
human		Sci Adv; The FLRT3-UNC5B checkpoint pathway inhibits T cell-based cancer immunotherapies; Authors - Kushal Prajapati