LRP2-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

LRP2-Specific Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(18)

Rezensionen (2)

Kat-Nr. : 19700-1-AP

Synonyme

LRP2, LRP 2, Low-density lipoprotein receptor-related protein 2, gp330, Glycoprotein 330

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  WB analysis using 19700-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19700-1-AP (LRP2-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 19700-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19700-1-AP (LRP2-Specific antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 19700-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19700-1-AP (LRP2-Specific antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 19700-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19700-1-AP (LRP2-Specific antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 19700-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19700-1-AP (LRP2-Specific antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human kidney using 19700-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using LRP2-Specific antibody (19700-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human kidney using 19700-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using LRP2-Specific antibody (19700-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Nierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:4000-1:16000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 6 publications below

Produktinformation

19700-1-AP bindet in WB, IHC, IF-P, ELISA LRP2-Specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: low density lipoprotein-related protein 2
• Berechnetes Molekulargewicht: 522 kDa
• Beobachtetes Molekulargewicht: 522 kDa
• GenBank-Zugangsnummer: NM_004525
• Gene symbol: LRP2
• Gene ID (NCBI): 4036
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LRP2, also named as gp330 and Megalin, belongs to the LDLR family. It acts together with cubilin to mediate HDL endocytosis. LRP2 may participate in regulation of parathyroid hormone and para-thyroid-hormone-related protein release. Defects in LRP2 are the cause of Donnai-Barrow syndrome (DBS).

Publikationen

Species	Application	Title
mouse	WB	J Hazard Mater; Epigenetic reprogramming of HDAC2 in CA1 excitatory neurons determines Pb-induced non-spatial memory deficits; Authors - Mengmeng Wang
mouse	IF	ACS Appl Mater Interfaces; Renal-Targeted Drug Delivery by Chitosan Oligosaccharide Micelles with HSA-Enriched Protein Corona for the Treatment of Ischemia/Reperfusion-Induced Acute Kidney Injury; Authors - Simin Nan
mouse	IF	EMBO Rep; Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance.; Authors - Ye Chen
human	WB,IHC	Nephrol Dial Transplant; Reduced proximal tubular expression of protein endocytic receptors in proteinuria is associated with urinary receptor shedding.; Authors - Hiwa Fatah
mouse	WB	Development; Mouse early extra-embryonic lineages activate compensatory endocytosis in response to poor maternal nutrition.; Authors - Congshan Sun
mouse	IHC,IF	Metabolism; Sodium-glucose cotransporter 2 inhibition attenuates protein overload in renal proximal tubule via suppression of megalin O-GlcNacylation in progressive diabetic nephropathy.; Authors - Hitomi Otomo

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Brittney (Verified Customer) (11-29-2018)

Excellent antibody for IHC. Used acidic antigen retrieval on paraffin embedded mouse kidney sections. Developed with DAB substrate.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse Kidney Tissue

LRP2-Specific Antibody Immunohistochemistry, validation (1:100 dilution) in Mouse Kidney Tissue (Cat no:19700-1-AP)

FH

Brittney (Verified Customer) (11-29-2018)

Excellent antibody for IHC. Used acidic antigen retrieval and developed with DAB substrate.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse Kidney Tissue