LRPPRC Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

LRPPRC Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

29

Rezensionen (1)

Kat-Nr. : 21175-1-AP

Synonyme

130 kDa leucine rich protein, CLONE 23970, GP130, LRP 130, LRP130, LRPPRC, LSFC

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Galerie der Validierungsdaten

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  WB analysis using 21175-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21175-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 21175-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 21175-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21175-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 21175-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 21175-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 21175-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 21175-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 21175-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 21175-1-AP

  IP Result of anti-LRPPRC (IP:21175-1-AP, 5ug; Detection:21175-1-AP 1:1000) with HeLa cells lysate 1200ug.

• 

  IHC staining of human kidney using 21175-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21175-1-AP (LRPPRC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 21175-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21175-1-AP (LRPPRC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 21175-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21175-1-AP (LRPPRC antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 21175-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21175-1-AP (LRPPRC antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of U2OS using 21175-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 21175-1-AP (LRPPRC antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).

• 

  IF Staining of U2OS using 21175-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 21175-1-AP (LRPPRC antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).

• 

  IF Staining of HepG2 using 21175-1-AP

  Immunofluorescent analysis of HepG2 cells, using LRPPRC antibody 21175-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HEK-293 using 21175-1-AP

  Immunofluorescent analysis of HEK-293 cells using 21175-1-AP (LRPPRC antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 21175-1-AP

  Immunofluorescent analysis of Hela cells, using LRPPRC antibody 21175-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HEK-293 using 21175-1-AP

  Immunofluorescent analysis of 293 cells, using LRPPRC antibody 21175-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, HeLa-Zellen, HepG2-Zellen, Mausherzgewebe, Mauslebergewebe, Mausnierengewebe, Maus-Skelettmuskelgewebe, PC-12-Zellen, Rattenherzgewebe, Rattenlebergewebe, Rattennierengewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: U2OS-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 24 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 3 publications below

Produktinformation

21175-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA LRPPRC und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LRPPRC fusion protein Ag15452
• Vollständiger Name: leucine-rich PPR-motif containing
• Berechnetes Molekulargewicht: 1394 aa, 158 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC050311
• Gene symbol: LRPPRC
• Gene ID (NCBI): 10128
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LRPPRC (also called LRP130 or GP130) is a 130-kDa RNA-binding protein of the pentatricopeptide repeat family. LRPPRC localizes primarily to the mitochondria where it binds to poly(A) mRNA. It plays a role in the translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. LRPPRC has also been shown to regulate nuclear gene transcription and to bind specific RNA molecules in both the nucleus and the cytoplasm. Mutations in the LRPPRC gene are associated with the French-Canadian type of Leigh syndrome.

Publikationen

Species	Application	Title
mouse	IP	Nat Biotechnol; Translation efficiency of mRNAs is increased by antisense oligonucleotides targeting upstream open reading frames.; Authors - Xue-Hai Liang
mouse	IF,WB,CoIP	Nat Commun; Mitochondrial membrane proteins and VPS35 orchestrate selective removal of mtDNA; Authors - Ayesha Sen
human	WB,IF	Nat Commun; A mosquito salivary protein promotes flavivirus transmission by activation of autophagy.; Authors - Peng Sun
human	WB	Genome Biol; Balanced mitochondrial and cytosolic translatomes underlie the biogenesis of human respiratory complexes; Authors - Iliana Soto ; KO Validated
human	IHC,CoIP	Redox Biol; LRPPRC regulates redox homeostasis via the circANKHD1/FOXM1 axis to enhance bladder urothelial carcinoma tumorigenesis.; Authors - Wen-Su Wei
human	WB	EMBO J; Functional screen reveals essential roles of miR-27a/24 in differentiation of embryonic stem cells.; Authors - Yanni Ma

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Angela (Verified Customer) (10-09-2018)

Worked well with 20 ug whole cell lysate run on 7% Tris-Acetate SDS-PAGE. Clean band seen between 120-150 kDa.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:6000
• Cell Tissue Type: HEK293 cells

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