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  • KD/KO Validated

LRPPRC Polyklonaler Antikörper

LRPPRC Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(45)

Kat-Nr. : 21175-1-AP

Synonyme

LRP130, LRP 130, Leucine-rich PPR motif-containing protein, mitochondrial, GP130, 130 kDa leucine-rich protein



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHCT 116-Zellen, HeLa-Zellen, HepG2-Zellen, Mausherzgewebe, Mausnierengewebe, Mauslebergewebe, Maus-Skelettmuskelgewebe, PC-12-Zellen, Rattenherzgewebe, Rattennierengewebe, Rattenlebergewebe, SH-SY5Y-Zellen
Erfolgreiche IPHeLa-Zellen
Erfolgreiche Detektion in IHChumanes Nierengewebe, humanes Herzgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCU2OS-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

21175-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA LRPPRC und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen LRPPRC fusion protein Ag15452
Vollständiger Name leucine-rich PPR-motif containing
Berechnetes Molekulargewicht 1394 aa, 158 kDa
Beobachtetes Molekulargewicht 130 kDa
GenBank-ZugangsnummerBC050311
Gene symbol LRPPRC
Gene ID (NCBI) 10128
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LRPPRC (also called LRP130 or GP130) is a 130-kDa RNA-binding protein of the pentatricopeptide repeat family. LRPPRC localizes primarily to the mitochondria where it binds to poly(A) mRNA. It plays a role in the translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. LRPPRC has also been shown to regulate nuclear gene transcription and to bind specific RNA molecules in both the nucleus and the cytoplasm. Mutations in the LRPPRC gene are associated with the French-Canadian type of Leigh syndrome.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for LRPPRC antibody 21175-1-APProtokoll herunterladen
IHC protocol for LRPPRC antibody 21175-1-APProtokoll herunterladenl
IF protocol for LRPPRC antibody 21175-1-APProtokoll herunterladen
IP protocol for LRPPRC antibody 21175-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseIP

Nat Biotechnol

Translation efficiency of mRNAs is increased by antisense oligonucleotides targeting upstream open reading frames.

Authors - Xue-Hai Liang
WB

Sci Adv

ARMC1 partitions between distinct complexes and assembles MIRO with MTFR to control mitochondrial distribution

Authors - Michael J McKenna
mouseIF,WB,CoIP

Nat Commun

Mitochondrial membrane proteins and VPS35 orchestrate selective removal of mtDNA

Authors - Ayesha Sen
humanWB,IF

Nat Commun

A mosquito salivary protein promotes flavivirus transmission by activation of autophagy.

Authors - Peng Sun
humanWB

Genome Biol

Balanced mitochondrial and cytosolic translatomes underlie the biogenesis of human respiratory complexes

Authors - Iliana Soto
  • KO Validated
humanIHC,CoIP

Redox Biol

LRPPRC regulates redox homeostasis via the circANKHD1/FOXM1 axis to enhance bladder urothelial carcinoma tumorigenesis.

Authors - Wen-Su Wei

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Manon (Verified Customer) (09-25-2025)

Clear band around 130 kDa

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Fibroblasts
FH

Angela (Verified Customer) (10-09-2018)

Worked well with 20 ug whole cell lysate run on 7% Tris-Acetate SDS-PAGE. Clean band seen between 120-150 kDa.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:6000
  • Cell Tissue Type: HEK293 cells