LRSAM1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

LRSAM1 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 24666-1-AP

Synonyme

hTAL, LRSAM1, RIFLE, TAL, Tsg101 associated ligase

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 24666-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24666-1-AP (LRSAM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 24666-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24666-1-AP (LRSAM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 24666-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24666-1-AP (LRSAM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 24666-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 24666-1-AP (LRSAM1 Antibody) at dilution of 1:50.

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  IHC staining of human skeletal muscle using 24666-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 24666-1-AP (LRSAM1 Antibody) at dilution of 1:50.

• 

  IF Staining of A431 using 24666-1-AP

  Immunofluorescent analysis of A431 cells using 24666-1-AP (LRSAM1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HepG2-Zellen, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below

Produktinformation

24666-1-AP bindet in WB, IHC, IF, ELISA LRSAM1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LRSAM1 fusion protein Ag20415
• Vollständiger Name: leucine rich repeat and sterile alpha motif containing 1
• Berechnetes Molekulargewicht: 723 aa, 84 kDa
• Beobachtetes Molekulargewicht: 84 kDa
• GenBank-Zugangsnummer: BC009239
• Gene symbol: LRSAM1
• Gene ID (NCBI): 90678
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LRSAM1 also named as hTAL, RIFLE orTsg101 associated ligase is a 723 amino acid protein, which contains 6 LRR repeats, 1 Ring-type zinc finger and 1 SAM domain. LRSAM1 is localizes in cytoplasm and highly expressed in adult spinal cord motoneurons as well as in fetal spinal cord and muscle tissue. As a E3 ubiquitin protein ligase, LRSAM1 mediates monoubiquitination of TSG101 at multiple sites, leading to inactivate the ability of TSG101 to sort endocytic and exocytic cargos, LRSAM1 gene mutation has an association with Charcot Maire Tooth disease 2P.

Publikationen

Species	Application	Title
human	WB	Cancer Cell Int; Leucine-rich repeat and sterile alpha motif containing 1 promotes the oncogenic growth of human hepatocellular carcinoma cells.; Authors - Lili Pian ; KD Validated
mouse	IF	Cell; Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules; Authors - Qinqin Cui
human	WB,IF	Autophagy; TRIM16-mediated lysophagy suppresses high-glucose-accumulated neuronal Aβ; Authors - Chang Woo Chae