LSG1 Polyklonaler Antikörper

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• KD/KO Validated

LSG1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 17750-1-AP

Synonyme

hLsg1, Large subunit GTPase 1 homolog, LSG1

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  WB analysis of HeLa using 17750-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17750-1-AP (LSG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17750-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17750-1-AP (LSG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17750-1-AP

  IP result of anti-LSG1 (IP:17750-1-AP, 4ug; Detection:17750-1-AP 1:500) with HeLa cells lysate 1400 ug.

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  IHC staining of human testis using 17750-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17750-1-AP (LSG1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 17750-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17750-1-AP (LSG1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 17750-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17750-1-AP (LSG1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 17750-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17750-1-AP (LSG1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 17750-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17750-1-AP (LSG1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 17750-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17750-1-AP (LSG1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 17750-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17750-1-AP (LSG1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 17750-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17750-1-AP (LSG1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 17750-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17750-1-AP (LSG1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 17750-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17750-1-AP (LSG1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 17750-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17750-1-AP (LSG1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 17750-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17750-1-AP (LSG1 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Nierengewebe, humanes Lungengewebe, humanes Eierstockgewebe, humanes Plazenta-Gewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

17750-1-AP bindet in WB, IHC, IF, IP, ELISA LSG1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LSG1 fusion protein Ag12016
• Vollständiger Name: large subunit GTPase 1 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 658 aa, 75 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC068500
• Gene symbol: LSG1
• Gene ID (NCBI): 55341
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IF	Aging Cell; Inhibition of the 60S ribosome biogenesis GTPase LSG1 causes endoplasmic reticular disruption and cellular senescence.; Authors - Asimina Pantazi ; KD Validated
human	WB	Mol Cell Biol; The Ribosome Assembly Factor LSG1 Interacts with Vesicle-Associated Membrane Protein-Associated Proteins (VAPs); Authors - Putri Sutjita
		EMBO Mol Med; Portimine A toxin causes skin inflammation through ZAKα-dependent NLRP1 inflammasome activation; Authors - Léana Gorse