LSS Polyklonaler Antikörper

LSS Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 13715-1-AP

Synonyme

hOSC, Lanosterol synthase, LSS, OSC

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Galerie der Validierungsdaten

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  WB analysis using 13715-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 13715-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13715-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 13715-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13715-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 13715-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13715-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13715-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13715-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13715-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 13715-1-AP

  IP Result of anti-LSS (IP:13715-1-AP, 3ug; Detection:13715-1-AP 1:500) with HepG2 cells lysate 2900ug.

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  IHC staining of human liver using 13715-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13715-1-AP (LSS Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 13715-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13715-1-AP (LSS Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse testis using 13715-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13715-1-AP (LSS Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse testis using 13715-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13715-1-AP (LSS Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 13715-1-AP

  Immunofluorescent analysis of HepG2 cells using 13715-1-AP (LSS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 13715-1-AP

  1X10^6 HeLa cells were stained with 0.2ug LSS antibody (13715-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-1376 cells, HeLa-Zellen, HepG2-Zellen, humanes Hirngewebe, Maushodengewebe, Mauslebergewebe, Maus-Pankreasgewebe, PC-3-Zellen, SH-SY5Y-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

13715-1-AP bindet in WB, IP, IHC, IF, FC, ELISA LSS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LSS fusion protein Ag4655
• Vollständiger Name: lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase)
• Berechnetes Molekulargewicht: 732 aa, 83 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC035638
• Gene symbol: LSS
• Gene ID (NCBI): 4047
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LSS, also named as OSC, is lanosterol synthase which catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus. LSS gene has three isoforms with MW 74 kDa and 82-85 kDa. This antibody can recognize all these three isoforms.

Publikationen

Species	Application	Title
mouse	WB	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
human	WB	J Invest Dermatol; Bi-allelic Variants in LSS Cause Palmoplantar Keratoderma-Congenital Alopecia Syndrome Type 2.; Authors - Fang Yang
human	WB,IHC	PLoS Genet; Metabolic and pathologic profiles of human LSS deficiency recapitulated in mice.; Authors - Yoichi Wada
rat	WB	J Biol Chem; The Endoplasmic Reticulum COPII-Vesicle Transport Machinery Coordinates Cellular Lipid Secretion and Cholesterol Biosynthesis.; Authors - Lee G D Fryer
human	WB, IHC	Phytomedicine; Hibiscus syriacus L. cultivated in callus culture exerts cytotoxicity in colorectal cancer via Notch signaling-mediated cholesterol biosynthesis suppression.; Authors - Xing Yue Xu
human	WB	J Biol Chem; The antiviral enzyme viperin inhibits cholesterol biosynthesis.; Authors - Timothy J Grunkemeyer