LY6D Polyklonaler Antikörper

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• KD/KO Validated

LY6D Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Rezensionen (1)

Kat-Nr. : 17361-1-AP

Synonyme

E48, E48 antigen, Ly 6D, Ly-6D, Lymphocyte antigen 6D

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  WB analysis of A431 using 17361-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17361-1-AP (LY6D antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 17361-1-AP

  WB result of LY6D antibody (17361-1-AP; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-LY6D transfected A431 cells.

• 

  IHC staining of human tonsillitis using 17361-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17361-1-AP (LY6D antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 17361-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17361-1-AP (LY6D Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 17361-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17361-1-AP (LY6D Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin cancer using 17361-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17361-1-AP (LY6D antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin cancer using 17361-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17361-1-AP (LY6D antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 6 publications below

Produktinformation

17361-1-AP bindet in WB, IHC, IF, ELISA LY6D und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LY6D fusion protein Ag10714
• Vollständiger Name: lymphocyte antigen 6 complex, locus D
• Berechnetes Molekulargewicht: 128 aa, 13 kDa
• Beobachtetes Molekulargewicht: 13-14 kDa
• GenBank-Zugangsnummer: BC031330
• Gene symbol: LY6D
• Gene ID (NCBI): 8581
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LY6D, also named as E48 and ThB, may act as a specification marker at earliest stage specification of lymphocytes between B- and T-cell development. It marks the earliest stage of B-cell specification.

Publikationen

Species	Application	Title
mouse,human	IF	Nat Commun; AP-1 and TGFß cooperativity drives non-canonical Hedgehog signaling in resistant basal cell carcinoma.; Authors - Catherine D Yao
mouse	IHC,IF	Cell Rep; Autocrine activation of MAPK signaling mediates intrinsic tolerance to androgen deprivation in LY6D prostate cancer cells; Authors - Ivana Steiner
mouse	WB	Diabetes; PMID: 28325853; Authors - Yu-Yao Zhang
mouse	IF	Elife; RUNX1 marks a luminal castration-resistant lineage established at the onset of prostate development.; Authors - Renaud Mevel
mouse,human	IHC,IF	Cancers (Basel); Lung Adenocarcinoma Mouse Models Based on Orthotopic Transplantation of Syngeneic Tumor-Initiating Cells Expressing EpCAM, SCA-1, and Ly6d.; Authors - Takashi Semba ; KD Validated
human	WB	PLoS One; Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer.; Authors - Kui Xiao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

James (Verified Customer) (05-23-2025)

This antibody did not work for my samples (liver protein extract). It gave unspecific bands over 130 kDa

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: liver cells

LY6D Antibody Western Blot validation (1/1000 dilution) in liver cells (Cat no:17361-1-AP)