LYPLA1 Polyklonaler Antikörper

LYPLA1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 16055-1-AP

Synonyme

Acyl protein thioesterase 1, APT 1, APT1, hAPT1, LPL I, LPL1, LYPLA1, Lysophospholipase 1, lysophospholipase I, LYSOPLA, LysoPLA I

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Galerie der Validierungsdaten

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  WB analysis using 16055-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16055-1-AP (LYPLA1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 16055-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16055-1-AP (LYPLA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16055-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16055-1-AP (LYPLA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16055-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16055-1-AP (LYPLA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 16055-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 16055-1-AP (LYPLA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16055-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16055-1-AP (LYPLA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16055-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16055-1-AP (LYPLA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 16055-1-AP

  IP Result of anti-LYPLA1 (IP:16055-1-AP, 4ug; Detection:16055-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 16055-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16055-1-AP (LYPLA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16055-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16055-1-AP (LYPLA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16055-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16055-1-AP (LYPLA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hirngewebe, humanes Lebergewebe, Maushirngewebe, Rattenhirngewebe, Rattenlebergewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 3 publications below

Produktinformation

16055-1-AP bindet in WB, IP, IHC, IF, ELISA LYPLA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LYPLA1 fusion protein Ag9022
• Vollständiger Name: lysophospholipase I
• Berechnetes Molekulargewicht: 230 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC010397
• Gene symbol: LYPLA1
• Gene ID (NCBI): 10434
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LYPLA1(Lysophospholipase 1) is also named as APT1(Acyl-protein thioesterase 1), LPL1 and belongs to the AB hydrolase 2 family. It is an important enzyme responsible for depalmitoylation of palmitoyl proteins and works as a thioesterase that cleaves the S-palmitoyl group of H-Ras, heterotrimeric G protein alfa subunit (Ga) , regulator of G protein signaling 4 (RGS4) and the endothelial isoform of nitric oxide synthase (eNOS) in vitro(PMID:19439193).

Publikationen

Species	Application	Title
human	IHC	J Transl Med; Identifying prognostic markers in spatially heterogeneous breast cancer microenvironment; Authors - Guohong Liu
human	WB	Ecotoxicol Environ Saf; PM2.5 promoted lipid accumulation in macrophage via inhibiting JAK2/STAT3 signaling pathways and aggravating the inflammatory reaction.; Authors - Liwei Yang
human	WB	PLoS One; Development of an activity-based probe for acyl-protein thioesterases.; Authors - Megan Garland
human	IHC	Drug Metab Dispos; Identification of Bioactivating Enzymes Involved in the Hydrolysis of Laninamivir Octanoate, a Long-acting Neuraminidase Inhibitor, in Human Pulmonary Tissue.; Authors - Kumiko Koyama
human	WB	Autophagy; Hypoxia-induced GPCPD1 depalmitoylation triggers mitophagy via regulating PRKN-mediated ubiquitination of VDAC1; Authors - Ying Liu
human	IHC,WB	Reprod Sci; Estrogen Regulates Scribble Localization in Endometrial Epithelial Cells Through Acyl Protein Thioesterase (APT)-Mediated S-Palmitoylation in Adenomyosis; Authors - Zhixing Jin