Lamin A/C Rekombinanter Antikörper

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• KD/KO Validated

Lamin A/C Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

5I16

Kat-Nr. : 81042-1-RR

Synonyme

LaminA/C, LMNA, LMN1, Lamin-A/C, lamin A

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  WB analysis using 81042-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81042-1-RR (Lamin A/C antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

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  WB analysis using 81042-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81042-1-RR (Lamin A/C antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 81042-1-RR

  WB result of Lamin A/C antibody (81042-1-RR; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Lamin A/C transfected HeLa cells.

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  IHC staining of human colon using 81042-1-RR

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 81042-1-RR (Lamin A/C antibody) at dilution of 1:12000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 81042-1-RR

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 81042-1-RR (Lamin A/C antibody) at dilution of 1:12000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 81042-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 81042-1-RR (Lamin A/C antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 81042-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 81042-1-RR (Lamin A/C antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 81042-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Lamin A/C antibody (81042-1-RR, Clone: 5I16 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  Affinity and Kinetic Characterization of 81042-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81042-1-RR against Human Lamin A/C were performed. The affinity constant is 92.9 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A431-Zellen, C6-Zellen, HeLa-Zellen, HUVEC-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen, SKOV-3-Zellen
• Erfolgreiche Detektion in IHC: Mausherzgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunhistochemie (IHC): IHC : 1:3000-1:12000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

81042-1-RR bindet in WB, IHC, IF/ICC, ELISA Lamin A/C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Lamin A/C fusion protein Ag0408
• Vollständiger Name: lamin A/C
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC003162
• Gene symbol: Lamin A/C
• Gene ID (NCBI): 4000
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lamin A/C is also named as LMNA, or LMN1. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. The lack of lamin A/C can be as a novel marker for undifferentiated embryonic stem cells and lamin A/C expression is as an early indicator of differentiation (PMID: 16179429). Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 74 kDa, 65 kDa, 70 kDa and 64 kDa. This antibody can recognize 4 isoforms of Lamin A/C.

Publikationen

Species	Application	Title
		Int J Mol Sci; Inflammation and Fibrosis in Progeria: Organ-Specific Responses in an HGPS Mouse Model; Authors - Peter Krüger
human	WB	Front Pharmacol; Rabeprazole suppressed gastric intestinal metaplasia through activation of GPX4-mediated ferroptosis; Authors - Jing Xie
mouse	WB,IF	Int J Mol Sci; Baricitinib and Lonafarnib Synergistically Target Progerin and Inflammation, Improving Lifespan and Health in Progeria Mice; Authors - Peter Krüger