Lin28B-specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Lin28B-specific Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(23)

Rezensionen (1)

Kat-Nr. : 16178-1-AP

Synonyme

LIN28B, Lin-28B, Lin28, Lin 28B, lin 28 homolog B (C. elegans)

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  IHC staining of human colon cancer using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16178-1-AP

  Immunofluorescent analysis of HepG2 cells using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Hirngewebe, humanes Nierengewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 12 publications below
• IF: See 3 publications below
• RIP: See 1 publications below

Produktinformation

16178-1-AP bindet in WB, IHC, IF/ICC, RIP, ELISA Lin28B-specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: lin-28 homolog B (C. elegans)
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: NM_001004317
• Gene symbol: LIN28B
• Gene ID (NCBI): 389421
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LIN28B, also named as CSDD2, belongs to the lin-28 family. It acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7). LIN2B is specifically expressed in embryonic muscle, neurons, and epithelia, as well as in embryonic carcinoma cells. However, it is suppressed in most adult tissues except for skeletal and cardiac muscle. LIN2B shares 78% sequence identity with LIN28A. This antibody is specific to LIN28B.

Publikationen

Species	Application	Title
human	WB	Cancer Res; Activation of the Lin28/let-7 Axis by Loss of ESE3/EHF Promotes a Tumorigenic and Stem-like Phenotype in Prostate Cancer.; Authors - Domenico Albino
human	WB	EMBO Rep; Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.; Authors - Yang Zhang ; KD Validated
human	IHC	Gastric Cancer; LOC101929709 promotes gastric cancer progression by aiding LIN28B to stabilize c-MYC mRNA; Authors - Tong-Peng Xu
human	WB	Mol Ther; PMID: 29456019; Authors - Xinyi Wang
rat	IHC	Sci Rep; Testicular expression of the Lin28/let-7 system: Hormonal regulation and changes during postnatal maturation and after manipulations of puberty.; Authors - S Sangiao-Alvarellos
Mouse	WB	Oncotarget; PMID: 29552298; Authors - Dawei Lei

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sarah (Verified Customer) (01-24-2023)

Antibody worked well

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: A172