Lysozyme Monoklonaler Antikörper

Lysozyme Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

CloneNo.

2G5A8

Kat-Nr. : 66456-1-Ig

Synonyme

LYZ, 1,4-beta-N-acetylmuramidase C, 2G5A8, EC:3.2.1.17, Lysozyme C

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  WB analysis of human spleen using 66456-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig spleen using 66456-1-Ig

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 66456-1-Ig

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66456-1-Ig

  LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 66456-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 66456-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66456-1-Ig (Lysozyme antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Milzgewebe, Hausschwein-Milzgewebe, Rattenmilzgewebe, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

66456-1-Ig bindet in WB, IHC, IF, ELISA Lysozyme und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Lysozyme fusion protein Ag6917
• Vollständiger Name: lysozyme (renal amyloidosis)
• Berechnetes Molekulargewicht: 17 kDa
• Beobachtetes Molekulargewicht: 15-17 kDa
• GenBank-Zugangsnummer: BC004147
• Gene symbol: Lysozyme
• Gene ID (NCBI): 4069
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LYZ(Lysozyme C) is also named as LZM and belongs to the glycosyl hydrolase 22 family. It catalyzes the hydrolysis of the bacterial cell wall beta(1-4) glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. In tissues and body fluids this protein is associated with the monocyte-macrophage system and enhance the activity of immunoagents. LYZ is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8). The full length protein has a signal peptide with 18 amino acid.

Publikationen

Species	Application	Title
mouse	WB	Cell Rep; Sox10 escalates vascular inflammation by mediating vascular smooth muscle cell transdifferentiation and pyroptosis in neointimal hyperplasia; Authors - Xin Xu
rat	IHC	J Pharmacol Sci; Oxymatrine alleviates NSAID-associated small bowel mucosal injury by regulating MIP-1/CCR1 signalling and gut microbiota; Authors - Ming Chen
human	IHC	Clin Transl Med; Single-cell transcriptome analysis reveals evolving tumour microenvironment induced by immunochemotherapy in nasopharyngeal carcinoma; Authors - Yaofei Jiang
human	WB,IF	Biomater Res; A New Light-Sensor System Affecting Cancer Cell Fate; Authors - Silvia Buonvino
mouse	WB	Res Sq; Sex-dependent effect of GPR109A gene deletion in myeloid cells on bone development in mice; Authors - Perry C Caviness