MACF1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MACF1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Rezensionen (1)

Kat-Nr. : 13058-1-AP

Synonyme

620 kDa actin binding protein, ABP620, ACF7, KIAA0465, KIAA1251, MACF, MACF1, Macrophin 1, OFC4, Trabeculin alpha

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Galerie der Validierungsdaten

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  WB analysis using 13058-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13058-1-AP (MACF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 13058-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 13058-1-AP (MACF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 13058-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 13058-1-AP (MACF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 13058-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13058-1-AP (MACF1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 13058-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13058-1-AP (MACF1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 13058-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13058-1-AP (MACF1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 13058-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13058-1-AP (MACF1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of RPE cells using 13058-1-AP

  IF results (golgi stain) of MACF1 antibody(13058-1-AP) (1:100) with RPE cells (fixed with 4% formaldehyde) by O’Neil Wiggan, Colorado State Univ.

• 

  IF Staining of NIH/3T3 using 13058-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using MACF1 antibody (13058-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of U-251 using 13058-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using MACF1 antibody (13058-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-87 MG-Zellen, Mauslungengewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungengewebe, humanes Herzgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: RPE-Zellen, NIH/3T3-Zellen, U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below

Produktinformation

13058-1-AP bindet in WB, IHC, IF, ELISA MACF1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MACF1 fusion protein Ag3730
• Vollständiger Name: microtubule-actin crosslinking factor 1
• Berechnetes Molekulargewicht: 5430 aa, 620 kDa
• Beobachtetes Molekulargewicht: 600 kD
• GenBank-Zugangsnummer: BC007330
• Gene symbol: MACF1
• Gene ID (NCBI): 23499
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MACF1, also called ACF7 (actin crosslinking family 7), is a member of the spectraplakin family and is a ubiquitously expressed 614 kDa protein. This protein facilitates actin-microtubule interactions at the cell periphery and couples the microtubule network to cellular junctions. It displays a conserved role in membrane protrusion formation during evolution. The two major MACF1 isoforms characterized to date, MACF1a and MACF1b, result from alternative splicing. Both proteins contain N-terminal actin-binding domains (ABD), followed by plakin domains, extended spectrin repeat domains, EF-hand motifs, and C-terminal microtubule-binding domains (MTBDs). MACF1 is expressed in numerous tissue, including the skin, brain, skeletal muscle, and heart.

Publikationen

Species	Application	Title
human	IHC	Gastroenterology; Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors; Authors - Mingjun Sun
human	WB	Nat Commun; The genomic landscape of cholangiocarcinoma reveals the disruption of post-transcriptional modifiers.; Authors - Yaodong Zhang
mouse	WB	Cell Death Differ; MACF1 promotes osteoblast differentiation by sequestering repressors in cytoplasm.; Authors - Lifang Hu ; KD Validated
mouse	WB	PLoS Genet; BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment.; Authors - Ying Hsu
human	WB	Oncotarget; LRP6 promotes invasion and metastasis of colorectal cancer through cytoskeleton dynamics.; Authors - Qian Yao
human	IHC	Biomed Pharmacother; Proteomics-based identification of tumor relevant proteins in lung adenocarcinoma.; Authors - Xin Zhou

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joleen (Verified Customer) (06-03-2019)

MACF1 should be the top band but there were a lot of non specific bands as well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

MACF1 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:13058-1-AP)

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