MAD1 Polyklonaler Antikörper

MAD1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 18322-1-AP

Synonyme

hMAD1, HsMAD1, MAD1, MAD1 like protein 1, MAD1L1, PIG9, Tax binding protein 181, TP53I9, TXBP181

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Galerie der Validierungsdaten

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  WB analysis using 18322-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18322-1-AP (MAD1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 18322-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18322-1-AP (MAD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 18322-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 18322-1-AP (MAD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18322-1-AP

  IP Result of anti-MAD1L1 (IP:18322-1-AP, 5ug; Detection:18322-1-AP 1:1000) with HeLa cells lysate 880ug.

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  IHC staining of human brain using 18322-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18322-1-AP (MAD1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 18322-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18322-1-AP (MAD1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 18322-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18322-1-AP (MAD1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 18322-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18322-1-AP (MAD1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 18322-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18322-1-AP (MAD1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 18322-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18322-1-AP (MAD1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 18322-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18322-1-AP (MAD1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 18322-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18322-1-AP (MAD1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 18322-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18322-1-AP (MAD1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 18322-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18322-1-AP (MAD1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 18322-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18322-1-AP (MAD1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 18322-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18322-1-AP (MAD1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 18322-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18322-1-AP (MAD1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 18322-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18322-1-AP (MAD1 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of A431 using 18322-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MAD1 antibody (18322-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of SH-SY5Y using 18322-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 18322-1-AP (MAD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Hirngewebe, Raji-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Hodengewebe, humanes Milzgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 1 publications below

Produktinformation

18322-1-AP bindet in WB, IP, IHC, IF, ELISA MAD1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAD1 fusion protein Ag13171
• Vollständiger Name: MAD1 mitotic arrest deficient-like 1 (yeast)
• Berechnetes Molekulargewicht: 83 kDa
• Beobachtetes Molekulargewicht: 83 kDa
• GenBank-Zugangsnummer: BC009964
• Gene symbol: MAD1L1
• Gene ID (NCBI): 8379
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAD1L1 is a component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. It recruits MAD2L1 to unattached kinetochores. Also it is required for anchoring MAD2L1 to the nuclear periphery. In addition, it binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding [PMID:10049595, 12837246].

Publikationen

Species	Application	Title
human	WB,IF	Nucleic Acids Res; ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.; Authors - Fengjie Yuan
mouse	WB	Cell Cycle; Nuclear interactor of ARF and Mdm2 regulates multiple pathways to activate p53.; Authors - Sara M Reed
human	WB	BMB Rep; TCP10L synergizes with MAD1 in transcriptional suppression and cell cycle arrest through mutual interaction.; Authors - Suqin Shen
human	WB	Sci Adv; Biallelic germline mutations in MAD1L1 induce a syndrome of aneuploidy with high tumor susceptibility; Authors - Carolina Villarroya-Beltri
mouse,human	WB	Aging (Albany NY); The HNF4A-CHPF pathway promotes proliferation and invasion through interactions with MAD1L1 in glioma; Authors - Haitao Luo