MAD2L1 Polyklonaler Antikörper

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• KD/KO Validated

MAD2L1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

20

Kat-Nr. : 10337-1-AP

Synonyme

HSMAD2, MAD2, MAD2 like protein 1, MAD2L1

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Galerie der Validierungsdaten

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  WB analysis using 10337-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10337-1-AP (MAD2L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 10337-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10337-1-AP (MAD2L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10337-1-AP

  WB result of MAD2L1 antibody (10337-1-AP, 1:500) with si-control and si-MAD2L1 transfected HEK293 cells.

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  WB analysis of rat liver using 10337-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10337-1-AP (MAD2L1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10337-1-AP

  IP Result of anti-MAD2L1 (IP:10337-1-AP, 3ug; Detection:10337-1-AP 1:500) with HEK-293 cells lysate 1800ug.

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  IHC staining of human liver cancer using 10337-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 25x lens).

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  IHC staining of human ovary tumor using 10337-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 10337-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 10337-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 10337-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary tumor using 10337-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 10337-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MAD2L1 antibody (10337-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HEK-293 using 10337-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 10337-1-AP (MAD2L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Daudi-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Raji-Zellen, Rattenlebergewebe, THP-1-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 12 publications below
• IHC: See 4 publications below
• IF: See 4 publications below

Produktinformation

10337-1-AP bindet in WB, IP, IHC, IF, ELISA MAD2L1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAD2L1 fusion protein Ag0307
• Vollständiger Name: MAD2 mitotic arrest deficient-like 1 (yeast)
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC000356
• Gene symbol: MAD2L1
• Gene ID (NCBI): 4085
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1, synonyms: MAD2, HSMAD2) is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is localized to human chromosome band 5q23.3 by in situ hybridization.

Publikationen

Species	Application	Title
	WB	Sci Adv; The cohesin release factor Wapl interacts with Bub3 to govern SAC activity in female meiosis I.; Authors - Changyin Zhou
human	WB,IF	Nucleic Acids Res; ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.; Authors - Fengjie Yuan
	IF	J Cell Biol; Loss of centromeric RNA activates the spindle assembly checkpoint in mammalian female meiosis I.; Authors - Tianyu Wu
human	WB	Oncogene; KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells.; Authors - Zhi-Chuan Zhu
	WB,IHC	Cancer Res; Deficiency of pRb family proteins and p53 in invasive urothelial tumorigenesis.; Authors - He Feng F
		Cancer Res; MPHOSPH1: a potential therapeutic target for hepatocellular carcinoma.; Authors - Xinran Liu