MAD2L1 Monoklonaler Antikörper

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• KD/KO Validated

MAD2L1 Monoklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

2F5F12

Kat-Nr. : 66014-1-Ig

Synonyme

HSMAD2, MAD2, MAD2 like protein 1, MAD2L1

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 66014-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66014-1-Ig

  WB result of MAD2L1 antibody (66014-1-Ig, 1:2000) with si-control and si-MAD2L1 transfected HEK293 cells.

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  WB analysis of HeLa using 66014-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66014-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 66014-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66014-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 66014-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis slide using 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:50.

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  IHC staining of human testis using 66014-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis slide using 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HEK-293
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66014-1-Ig bindet in WB, IHC, ELISA MAD2L1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MAD2L1 fusion protein Ag17512
• Vollständiger Name: MAD2 mitotic arrest deficient-like 1 (yeast)
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC000356
• Gene symbol: MAD2L1
• Gene ID (NCBI): 4085
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1, synonyms: MAD2, HSMAD2) is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is localized to human chromosome band 5q23.3 by in situ hybridization.

Publikationen

Species	Application	Title
human	WB	FEBS Open Bio; SETDB2 interacts with BUBR1 to induce accurate chromosome segregation independently of its histone methyltransferase activity; Authors - Yanhong Tu