MAGED1 Polyklonaler Antikörper

MAGED1 Polyklonal Antikörper für FC, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 11539-1-AP

Synonyme

DLXIN 1, MAGE D1 antigen, MAGE tumor antigen CCF, MAGED1, melanoma antigen family D, 1, Melanoma associated antigen D1, NRAGE, PP2250

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 11539-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11539-1-AP (MAGED1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11539-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11539-1-AP (MAGED1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11539-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11539-1-AP (MAGED1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Apoptosised HeLa using 11539-1-AP

  Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 11539-1-AP (MAGED1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11539-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11539-1-AP (MAGED1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 11539-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11539-1-AP (MAGED1 Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human gliomas using 11539-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11539-1-AP (MAGED1 Antibody) at dilution of 1:100 (under 40x lens).

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  FC experiment of HeLa using 11539-1-AP

  1X10^6 HeLa cells were stained with 0.2ug MAGED1 antibody (11539-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Apoptotisierte HeLa-Zellen, HeLa-Zellen, humanes Hirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

11539-1-AP bindet in WB, IP, IHC, IF, FC, ELISA MAGED1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAGED1 fusion protein Ag2109
• Vollständiger Name: melanoma antigen family D, 1
• Berechnetes Molekulargewicht: 86 kDa; 92 kDa
• Beobachtetes Molekulargewicht: 66 kDa, 92-95 kDa
• GenBank-Zugangsnummer: BC014070
• Gene symbol: MAGED1
• Gene ID (NCBI): 9500
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAGED1 (also known as NRAGE or Dlxin-1) is a member of the type II melanoma antigen (MAGE) family of protein. MAGED1 can interact with the nerve growth factor receptor p75NTR, antagonize the association of p75NTR with TrkA, inhibit cell cycle progression, and facilitate p75NTR-mediated apoptosis (PMID: 10985348). It has been reported that MAGED1 binds Dlx5 and regulates its transcriptional function. In vivo, MAGED1 binds not only Dlx5 but also Dlx7 and Msx2 and forms homomultimers (PMID: 11084035). MAGED1 knockout mice show depressive behavior and impairments of circadian rhythm (PMID: 20300063; 23314527).

Publikationen

Species	Application	Title
rat	WB,IHC,IF,IP	Sci Rep; Dynamic expression of Mage-D1 in rat dental germs and potential role in mineralization of ectomesenchymal stem cells; Authors - Meng Li