MAN1B1 Monoklonaler Antikörper

MAN1B1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

3C9F10

Kat-Nr. : 68121-1-Ig

Synonyme

ER alpha 1,2 mannosidase, ER mannosidase 1, ERMan1, MAN1B1, MANA ER

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 68121-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 68121-1-Ig (MAN1B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68121-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68121-1-Ig (MAN1B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68121-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68121-1-Ig (MAN1B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HaCaT-Zellen, HEK-293-Zellen, HepG2-Zellen, LNCaP-Zellen, MCF-7-Zellen, U-87 MG-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68121-1-Ig bindet in WB, ELISA MAN1B1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MAN1B1 fusion protein Ag28302
• Vollständiger Name: mannosidase, alpha, class 1B, member 1
• Berechnetes Molekulargewicht: 80 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC002953
• Gene symbol: MAN1B1
• Gene ID (NCBI): 11253
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAN1B1, also known as ERMAN1, MANA-ER, belongs to the glycosyl hydrolase 47 family. MAN1B1 is involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation, specifically converting Man9GlcNAc to Man8GlcNAc isomer B. At high enzyme concentrations, it further trims the carbohydrates to Man5-6GlcNAc2 (PMID: 18003979). Mutations in MAN1B1 cause autosomal-recessive intellectual disability. This gene has been associated with Rafiq syndrome (PMID: 21763484).