MAP2 Monoklonaler Antikörper

MAP2 Monoklonal Antikörper für FC, IF, IHC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

17

CloneNo.

1C3E6

Rezensionen (2)

Kat-Nr. : 67015-1-Ig

Synonyme

MAP 2, MAP2, MAP2A, MAP2B, MAP2C

---

Galerie der Validierungsdaten

• 

  IHC staining of mouse brain using 67015-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67015-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67015-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67015-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 67015-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 67015-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 67015-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 67015-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 67015-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67015-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67015-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 67015-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 67015-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 67015-1-Ig (MAP2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 67015-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using MAP2 antibody (67015-1-Ig, Clone: 1C3E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 67015-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAP2 antibody (67015-1-Ig, Clone: 1C3E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 67015-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAP2 antibody (67015-1-Ig, Clone: 1C3E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of SH-SY5Y using 67015-1-Ig

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human MAP2 (67015-1-Ig, Clone:1C3E6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Neuro-2a using 67015-1-Ig

  1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human MAP2 (67015-1-Ig, Clone:1C3E6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hirngewebe, Maus-Cerebellum-Gewebe, Ratten-Cerebellum-Gewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Rattenhirngewebe, Maushirngewebe
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen, Neuro-2a-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 15 publications below
• IP: See 1 publications below

Produktinformation

67015-1-Ig bindet in WB, IP, IHC, IF, FC, ELISA MAP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MAP2 fusion protein Ag11349
• Vollständiger Name: microtubule-associated protein 2
• Berechnetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: BC038857
• Gene symbol: MAP2
• Gene ID (NCBI): 4133
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAP2 (microtubule-associated protein 2) is a cytoskeleton protein abundant in brain and has important role in neuronal morphogenesis. Multiple high molecular weight (MW) and low molecular weight (MW) MAP2 isoforms are expressed within axons, dendrites, and cell bodies. The expression of MAP2 is regulated in both a tissue- and developmentally specific manner. MAP2 antibodies have been widely used to mark the neuron or dendrite formation.

Publikationen

Species	Application	Title
mouse	IF	Cell; Revisiting astrocyte to neuron conversion with lineage tracing in vivo.; Authors - Lei-Lei Wang
mouse	IF	Cell Stem Cell; In vivo reprogramming of NG2 glia enables adult neurogenesis and functional recovery following spinal cord injury.; Authors - Wenjiao Tai ; KO Validated
rat	IF	Small; Stemness Maintenance and Massproduction of Neural Stem Cells on Poly L-Lactic Acid Nanofibrous Membrane Based on Piezoelectriceffect.; Authors - Xiheng Lu
rat	IF	Cell Death Dis; ChemR23 activation attenuates cognitive impairment in chronic cerebral hypoperfusion by inhibiting NLRP3 inflammasome-induced neuronal pyroptosis; Authors - Yaxuan Zhang
mouse	WB,IF	Cell Death Dis; Pyruvate dehydrogenase kinase 1 protects against neuronal injury and memory loss in mouse models of diabetes; Authors - Yuan Yao
mouse	IF	Acta Neuropathol Commun; TMEM106B deficiency impairs cerebellar myelination and synaptic integrity with Purkinje cell loss.; Authors - Tuancheng Feng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Alessandro (Verified Customer) (12-09-2023)

good IF antibody. no unspecific staining

• Applications: Immunofluorescence
• Cell Tissue Type: Cortical organoids

FH

Azita (Verified Customer) (05-31-2021)

Human primary cortical cells went trough ICC and the MAP2 antibody at the 1/1000 dilution was used overnight at 4°C.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Human primary cortical cells

MAP2 Antibody Immunohistochemistry validation (1/1000 dilution) in Human primary cortical cells (Cat no:67015-1-Ig)

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C