MAP2K4 Monoklonaler Antikörper

MAP2K4 Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E4C1

Kat-Nr. : CL488-67333

Synonyme

MEK4, JNK-activating kinase 1, JNK activating kinase 1, EC:2.7.12.2, Dual specificity mitogen-activated protein kinase kinase 4

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  IF Staining of HepG2 using CL488-67333

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 MAP2K4 antibody (CL488-67333, Clone: 2E4C1 ) at dilution of 1:200.

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  FC experiment of HepG2 using CL488-67333

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MAP2K4 (CL488-67333, Clone:2E4C1) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67333 bindet in IF/ICC, FC (Intra) MAP2K4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MAP2K4 fusion protein Ag11348
• Vollständiger Name: mitogen-activated protein kinase kinase 4
• Berechnetes Molekulargewicht: 399 aa, 44 kDa
• Beobachtetes Molekulargewicht: 44-50 kDa
• GenBank-Zugangsnummer: BC036032
• Gene symbol: MAP2K4
• Gene ID (NCBI): 6416
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MEK4 is also named as JNKK1, MAP2K4 , MKK4, PRKMK4, SEK1, SERK1, SKK1 and belongs to the protein kinase superfamily. MEK4 is a member of the mitogen-activated protein kinase family, originally identified as a kinase involved in the stress-activated protein kinase pathway by directly phosphorylating c-Jun NH 2-terminal kinase. This protein has 2 isoforms produced by alternative splicing.