MAP3K1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MAP3K1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 19970-1-AP

Synonyme

MAP3K1, MAPK/ERK kinase kinase 1, MAPKKK1, MEK kinase 1, MEKK, MEKK 1, MEKK1

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Galerie der Validierungsdaten

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  WB analysis using 19970-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19970-1-AP (MAP3K1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 19970-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19970-1-AP (MAP3K1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 19970-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19970-1-AP (MAP3K1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 19970-1-AP

  IP result of anti-MAP3K1(IP:19970-1-AP, 4ug; Detection:19970-1-AP 1:1000) with NIH/3T3 cells lysate 1120 ug.

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  IHC staining of human spleen using 19970-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19970-1-AP (MAP3K1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human spleen using 19970-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19970-1-AP (MAP3K1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 19970-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 19970-1-AP (MAP3K1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausembryogewebe, A431-Zellen, A549-Zellen, Mausmilzgewebe, NIH/3T3-Zellen, Rattenmilzgewebe
• Erfolgreiche IP: NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IF: See 1 publications below

Produktinformation

19970-1-AP bindet in WB, IP, IHC, IF, ELISA MAP3K1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase kinase kinase 1
• Berechnetes Molekulargewicht: 164 kDa
• Beobachtetes Molekulargewicht: 164 kDa
• GenBank-Zugangsnummer: NM_005921
• Gene symbol: MAP3K1
• Gene ID (NCBI): 4214
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAP3K1, also named as MAPKKK1, MEKK and MEKK1, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase kinase subfamily. MAP3K1 is a component of a protein kinase signal transduction cascade. MAP3K1 activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. It activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway. It catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody recognizes the N-term of MAP3K1.

Publikationen

Species	Application	Title
human	WB	J Immunol Res; Circulating miR-320b Contributes to CD4+ T-Cell Proliferation in Systemic Lupus Erythematosus via MAP3K1; Authors - Zutong Li
human	WB	Int J Biol Sci; LncRNA SLCO4A1-AS1 predicts poor prognosis and promotes proliferation and metastasis via the EGFR/MAPK pathway in colorectal cancer.; Authors - Rui Tang
human	WB	Int Immunopharmacol; Extracellular vesicles secreted from mesenchymal stem cells exert anti-apoptotic and anti-inflammatory effects via transmitting microRNA-18b in rats with diabetic retinopathy.; Authors - Zepeng Xu
mouse	WB,IF	Biomed Pharmacother; Xihuang pill promotes apoptosis of Treg cells in the tumor microenvironment in 4T1 mouse breast cancer by upregulating MEKK1/SEK1/JNK1/AP-1 pathway.; Authors - Liang Su
human	WB	Cell Death Dis; LncRNA KCNQ1OT1 activated by c-Myc promotes cell proliferation via interacting with FUS to stabilize MAP3K1 in acute promyelocytic leukemia.; Authors - Doudou Tang
mouse	WB	Clin Sci (Lond); USP4 deficiency exacerbates hepatic ischaemia/reperfusion injury via TAK1 signalling.; Authors - Jiangqiao Zhou