MLK2 Polyklonaler Antikörper

MLK2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 19974-1-AP

Synonyme

MAP3K10, EC:2.7.11.25, Mitogen-activated protein kinase kinase kinase 10, Mixed lineage kinase 2, MST

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  WB analysis of human skeletal muscle using 19974-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19974-1-AP (MLK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 19974-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19974-1-AP (MLK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 19974-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19974-1-AP (MLK2 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 19974-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19974-1-AP (MLK2 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 19974-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19974-1-AP (MLK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Skelettmuskelgewebe, humanes Hirngewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

19974-1-AP bindet in WB, IHC, ELISA MLK2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase kinase kinase 10
• Berechnetes Molekulargewicht: 104 kDa
• Beobachtetes Molekulargewicht: 104-115 kDa
• GenBank-Zugangsnummer: NM_002446
• Gene symbol: MAP3K10
• Gene ID (NCBI): 4294
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAP3K10, also named as MLK2 and MST, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase kinase subfamily. MAP3K10 activates the JUN N-terminal pathway. MAP3K10 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody recognizes the N-term of MAP3K10. The antibody has no cross reaction to MAP3K9.

Publikationen

Species	Application	Title
human	WB	Lab Chip; Paper-based microreactor array for rapid screening of cell signaling cascades.; Authors - Chia-Hao Huang
human	WB	J Biol Chem; Phosphorylation of mixed lineage kinase MLK3 by cyclin-dependent kinases CDK1 and CDK2 controls ovarian cancer cell division.; Authors - Luis Cedeno-Rosario