MLK3 Polyklonaler Antikörper

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• KD/KO Validated

MLK3 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 11996-1-AP

Synonyme

MAP3K11, Mixed lineage kinase 3, MLK 3, MLK3, PTK1, SPRK

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Galerie der Validierungsdaten

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  WB analysis using 11996-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11996-1-AP (MLK3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11996-1-AP

  WB result of MLK3 antibody (11996-1-AP, 1:500) with si-Control and si-MLK3 transfected HEK293 cells.

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  IHC staining of human colon cancer using 11996-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11996-1-AP (MLK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11996-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11996-1-AP (MLK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11996-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11996-1-AP (MLK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11996-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11996-1-AP (MLK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 11996-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using MLK3 antibody (11996-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 11996-1-AP

  Immunofluorescent analysis of Hela cells, using MAP3K11 antibody 11996-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, C6-Zellen, HEK-293-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: NIH/3T3-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

11996-1-AP bindet in WB, IP, IHC, IF, ELISA MLK3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MLK3 fusion protein Ag2636
• Vollständiger Name: mitogen-activated protein kinase kinase kinase 11
• Berechnetes Molekulargewicht: 847 aa, 93 kDa
• Beobachtetes Molekulargewicht: 93 and 65 kDa
• GenBank-Zugangsnummer: BC011263
• Gene symbol: MAP3K11
• Gene ID (NCBI): 4296
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MLK3 (Mixed lineage kinase 3) is also named as MAP3K11 and activates the JUN N-terminal pathway. It influences microtubule organization during the cell cycle. Mouse epididymal sperm contained an 60 kDa variant of MLK3, which may have been a result of proteolytic cleavage of the longer 93 kDa form seen in many tissues and the 50 kDa band is not known and not infrequently observed(PMID:17377140). Exposure of cells to LPS resulted in an increase in MLK3 dimers(of approximately 220 kDa), whereas inactivation of GSK-3b by TWS119 blocked MLK3 dimerization(PMID:21194439).

Publikationen

Species	Application	Title
mouse	WB	Acta Pharmacol Sin; Harmine is an effective therapeutic small molecule for the treatment of cardiac hypertrophy.; Authors - Jie Huang
human	WB	J Virol; High-throughput screening identifies mixed lineage kinase 3 as a key host regulatory factor in Zika virus infection.; Authors - Hua Xu
mouse	WB	J Ethnopharmacol; Xinyang Tablet inhibits MLK3-mediated pyroptosis to attenuate inflammation and cardiac dysfunction in pressure overload.; Authors - Junyan Wang
human	IP, WB, IF	Front Physiol; Ras-Related C3 Botulinum Toxin Substrate 1 Combining With the Mixed Lineage Kinase 3- Mitogen-Activated Protein Kinase 7- c-Jun N-Terminal Kinase Signaling Module Accelerates Diabetic Nephropathy.; Authors - Changjiang Ying
mouse	WB	J Mol Histol; Silence of MLK3 alleviates lipopolysaccharide-induced lung epithelial cell injury via inhibiting p53-mediated ferroptosis.; Authors - Xiangjun Chen
mouse	WB	Cell Death Dis; Pyroptosis and ferroptosis induced by mixed lineage kinase 3 (MLK3) signaling in cardiomyocytes are essential for myocardial fibrosis in response to pressure overload.; Authors - Junyan Wang