MAP4K3 Polyklonaler Antikörper

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• KD/KO Validated

MAP4K3 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 14702-1-AP

Synonyme

GLK, MAP4K3, MAPKKKK3, MEK kinase kinase 3, MEKKK 3, RAB8IPL1

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Galerie der Validierungsdaten

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  WB analysis of human skeletal muscle using 14702-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14702-1-AP (MAP4K3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14702-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14702-1-AP (MAP4K3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14702-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14702-1-AP (MAP4K3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 14702-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14702-1-AP (MAP4K3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skeletal muscle using 14702-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14702-1-AP (MAP4K3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 14702-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14702-1-AP (MAP4K3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 14702-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14702-1-AP (MAP4K3 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Skelettmuskelgewebe, HeLa-Zellen, humanes Hirngewebe
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:300-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

14702-1-AP bindet in WB, IHC, ELISA MAP4K3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAP4K3 fusion protein Ag6372
• Vollständiger Name: mitogen-activated protein kinase kinase kinase kinase 3
• Berechnetes Molekulargewicht: 101 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC071579
• Gene symbol: MAP4K3
• Gene ID (NCBI): 8491
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAP4K3(Mitogen-activated protein kinase kinase kinase kinase 3) has kinase activity and that it activates JNK but not MAPK1, MAPK3 or MAPK14 through the activation of MAP3K1 and MAP2K4. Endogenous MAP4K3 kinase activity could be stimulated by exposure to ultraviolet radiation or to tumor necrosis factor.

Publikationen

Species	Application	Title
mouse,rat	WB	Bioengineered; Mitogen-activating protein kinase kinase kinase kinase-3, inhibited by Astragaloside IV through H3 lysine 4 monomethylation, promotes the progression of diabetic nephropathy by inducing apoptosis.; Authors - Yuyan Fan ; KD Validated