MAP4K4 Polyklonaler Antikörper

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• KD/KO Validated

MAP4K4 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 55247-1-AP

Synonyme

FLH21957, HGK, HPK/GCK like kinase HGK, KIAA0687, MAP4K4, MEK kinase kinase 4, MEKKK 4, Nck interacting kinase, NIK

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 55247-1-AP

  WB result of MAP4K4 antibody (55247-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAP4K4 transfected Jurkat cells.

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  WB analysis of Jurkat using 55247-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55247-1-AP (MAP4K4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 55247-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 55247-1-AP (MAP4K4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 55247-1-AP

  IP result of anti-MAP4K4 (IP:55247-1-AP, 4ug; Detection:55247-1-AP 1:300) with Jurkat cells lysate 4000 ug.

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  IHC staining of human skeletal muscle using 55247-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55247-1-AP (MAP4K4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 55247-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55247-1-AP (MAP4K4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 55247-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55247-1-AP (MAP4K4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 55247-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55247-1-AP (MAP4K4 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 55247-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 55247-1-AP (MAP4K4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 55247-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 55247-1-AP (MAP4K4 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, PC-3-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe, humanes Lebergewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

55247-1-AP bindet in WB, IP, IHC, IF, ELISA MAP4K4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase kinase kinase kinase 4
• Berechnetes Molekulargewicht: 151 kDa
• Beobachtetes Molekulargewicht: 130-160 kDa
• GenBank-Zugangsnummer: NM_004834
• Gene symbol: MAP4K4
• Gene ID (NCBI): 9448
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAP4K4, also named as RAB8IPL1, GLK and MEKKK4, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. It may play a role in the response to environmental stress. MAP4K4 appears to act upstream of the JUN N-terminal pathway. This antibody is specific to MAP4K4.

Publikationen

Species	Application	Title
human	WB	Anal Chem; Parallel-Reaction-Monitoring-Based Proteome-Wide Profiling of Differential Kinase Protein Expression during Prostate Cancer Metastasis in Vitro.; Authors - Weili Miao
human	WB	EBioMedicine; Targeting miR-30d reverses pathological cardiac hypertrophy.; Authors - Jin Li
mouse	WB	J Proteome Res; Global Phosphoproteomic Analysis Reveals Significant Metabolic Reprogramming in the Termination of Liver Regeneration in Mice.; Authors - Jingzi Zhang
mouse	WB,IF	Neural Regen Res; MAP4K4 induces early blood-brain barrier damage in a murine subarachnoid hemorrhage model.; Authors - Zheng Zou ; KD Validated
human	IHC	PeerJ; Construction and validation of a novel prognostic signature of microRNAs in lung adenocarcinoma.; Authors - Wanzhen Li
human	WB	Pathol Res Pract; MiR-181c suppresses triple-negative breast cancer tumorigenesis by targeting MAP4K4.; Authors - Dan Xie