MAPK13 Monoklonaler Antikörper

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• KD/KO Validated

MAPK13 Monoklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

CloneNo.

1B12A6

Kat-Nr. : 60252-1-Ig

Synonyme

MAP kinase 13, MAP kinase p38 delta, MAPK 13, MAPK13, p38delta, PRKM13, SAPK4

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Galerie der Validierungsdaten

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  WB analysis of Ramos using 60252-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 60252-1-Ig (MAPK13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 60252-1-Ig

  WB result of MAPK13 antibody (60252-1-Ig, 1:2000) with si-Control and si-MAPK13 transfected HepG2 cells.

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  WB analysis of Daudi using 60252-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 60252-1-Ig (MAPK13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 60252-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60252-1-Ig (MAPK13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 60252-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60252-1-Ig (MAPK13 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 60252-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60252-1-Ig (MAPK13 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Ramos-Zellen, Daudi-Zellen, HEK-293-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

60252-1-Ig bindet in WB, IHC,ELISA MAPK13 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MAPK13 fusion protein Ag21240
• Vollständiger Name: mitogen-activated protein kinase 13
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 38-42 kDa
• GenBank-Zugangsnummer: BC000433
• Gene symbol: MAPK13
• Gene ID (NCBI): 5603
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAPK13(Mitogen-activated protein kinase 13) is also named as PRKM13, SAPK4 and belongs to the protein kinase superfamily. MAPK13 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2.