SIN1/MAPKAP1 Polyklonaler Antikörper

SIN1/MAPKAP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Rezensionen (2)

Kat-Nr. : 15463-1-AP

Synonyme

MAPKAP1, MIP1, Mitogen-activated protein kinase 2-associated protein 1, SAPK interacting protein 1, SIN1

---

• 

  WB analysis of mouse kidney using 15463-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15463-1-AP (MAPKAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15463-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15463-1-AP (MAPKAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 15463-1-AP

  IP result of anti-SIN1 (IP:15463-1-AP, 4ug; Detection:15463-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of mouse kidney using 15463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15463-1-AP (SIN1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 15463-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15463-1-AP (MAPKAP1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15463-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15463-1-AP (MAPKAP1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 15463-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SIN1 antibody (15463-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, HeLa-Zellen
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

15463-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SIN1/MAPKAP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SIN1/MAPKAP1 fusion protein Ag7753
• Vollständiger Name: mitogen-activated protein kinase associated protein 1
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC002326
• Gene symbol: MAPKAP1
• Gene ID (NCBI): 79109
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mitogen-activated protein kinase 2-associated protein 1 (MAPKAP1) is also named as MIP1 and SIN1. SIN1/MAPKAP1 is a major partner of mTORC2, acting as a scaffold and responsible for the substrate specificity of the mTOR catalytic subunit (PMID: 37877351). Besides as a component of MTORC2, MAPKAP1 also functions as MEKK2 and Ras-interacting protein and involves in regulating MEKK2 activation and RAS signaling (PMID: 31773361).

Publikationen

Species	Application	Title
mouse	IF	Cell Rep; Mitochondrial dynamics define muscle fiber type by modulating cellular metabolic pathways; Authors - Tatsuki Yasuda
human	WB	EMBO Rep; Nup358 restricts ER-mitochondria connectivity by modulating mTORC2/Akt/GSK3β signalling; Authors - Misha Kalarikkal ; KD Validated
mouse	WB	Cell Rep; Hepatic DNAJB9 Drives Anabolic Biasing to Reduce Steatosis and Obesity.; Authors - Fangfang Sun
mouse	WB	bioRxiv; Host-derived oxidized phospholipids initiate effector-triggered immunity fostering lethality upon microbial encounter; Authors - Marco Di Gioia
mouse	IF	bioRxiv; Ibudilast Protects Retinal Bipolar Cells from Excitotoxic Retinal Damage and Activates the mTOR Pathway; Authors - Sumaya Hamadmad
human	WB,IHC	Cell Signal; Overexpression of microRNA-611 inhibits TGF-β-induced epithelial-mesenchymal transition and migration in lung cancer cells through MAPKAP1; Authors - Bin Ke

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Shile (Verified Customer) (07-24-2025)

The SIN1 rabbit polyclonal antibody works well in our WB (1:500).

• Applications: Western Blot
• Primary Antibody Dilution: WB 1:500
• Cell Tissue Type: Human Rh30 cancer cell lysates

FH

Shile (Verified Customer) (01-31-2025)

15463-1-AP SIN1 Rabbit PolyAb was working well in our Western blotting (1:500).

• Applications: Western Blot
• Primary Antibody Dilution: 1:500 for WB
• Cell Tissue Type: 293T cell lysates

SIN1/MAPKAP1 Antibody Western Blot validation (1:500 for WB dilution) in 293T cell lysates (Cat no:15463-1-AP)