EB1 Polyklonaler Antikörper

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• KD/KO Validated

EB1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 17717-1-AP

Synonyme

MAPRE1, Microtubule-associated protein RP/EB family member 1, End-binding protein 1, End binding protein 1, APC-binding protein EB1

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  WB analysis of HeLa using 17717-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17717-1-AP (MAPRE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17717-1-AP

  WB result of EB1 antibody (17717-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EB1 transfected HeLa cells.

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  WB analysis of A431 using 17717-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17717-1-AP (MAPRE1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17717-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17717-1-AP (MAPRE1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17717-1-AP

  IP result of anti-EB1 (IP:17717-1-AP, 4ug; Detection:17717-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IP experiment of mouse thymus using 17717-1-AP

  IP result of anti-EB1 (IP:17717-1-AP, 4ug; Detection:17717-1-AP 1:1000) with mouse thymus tissue lysate 2800ug.

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  IP experiment of Caco-2 using 17717-1-AP

  IP result of anti-EB1 (IP:17717-1-AP, 4ug; Detection:17717-1-AP 1:1000) with Caco-2 cells lysate 1920 ug.

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  IHC staining of human kidney using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human colon using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 17717-1-AP (EB1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 17717-1-AP (EB1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17717-1-AP (EB1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17717-1-AP (EB1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 17717-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EB1 antibody (17717-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of HeLa using 17717-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human EB1 (17717-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, Jurkat-Zellen
• Erfolgreiche IP: HeLa-Zellen, Caco-2-Zellen, Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Kolonkarzinomgewebe, humanes Lungengewebe, humanes Plazenta-Gewebe, humanes Hautgewebe, humanes Milzgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 4 publications below

Produktinformation

17717-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA EB1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EB1 fusion protein Ag12092
• Vollständiger Name: microtubule-associated protein, RP/EB family, member 1
• Berechnetes Molekulargewicht: 268 aa, 30 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC106068
• Gene symbol: EB1
• Gene ID (NCBI): 22919
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human		Nat Commun; Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.; Authors - Hai-Qing Tu
human	IF	Elife; α-/γ-Taxilin are required for centriolar subdistal appendage assembly and microtubule organization.; Authors - Dandan Ma
mouse	IF	Biol Open; MACF1 promotes preosteoblast migration by mediating focal adhesion turnover through EB1; Authors - Peihong Su
mouse	IF	Biol Open; MACF1 promotes preosteoblast migration by mediating focal adhesion turnover through EB1.; Authors - Peihong Su
human	IF	Molecules; Pyrrolyldihydropyrazino[1,2-a]indoletrione Analogue Microtubule Inhibitor Induces Cell-Cycle Arrest and Apoptosis in Colorectal Cancer Cells; Authors - Dong-Lin Yang