MAT2A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MAT2A Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(21)

Kat-Nr. : 55309-1-AP

Synonyme

AMS2, EC:2.5.1.6, MAT 2, MATA2, MATII

---

• 

  WB analysis using 55309-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55309-1-AP (MAT2A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55309-1-AP

  WB result of MAT2A antibody (55309-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-control and sh-MAT2A transfected HeLa cells.

• 

  IP experiment of HepG2 using 55309-1-AP

  IP result of anti-MAT2A (IP:55309-1-AP, 4ug; Detection:55309-1-AP 1:500) with HepG2 cells lysate 3200ug.

• 

  IHC staining of human stomach cancer using 55309-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 55309-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 55309-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 55309-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 55309-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 55309-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 55309-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MAT2A antibody (55309-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Kolonkarzinomgewebe, humanes Nierengewebe, Mauslebergewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 18 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Produktinformation

55309-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA MAT2A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: methionine adenosyltransferase II, alpha
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 47 kDa, 49 kDa
• GenBank-Zugangsnummer: NM_005911
• Gene symbol: MAT2A
• Gene ID (NCBI): 4144
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAT2A, also named as AMS2, MATA2 and AdoMet synthase 2, belongs to the AdoMet synthase family. It catalyzes the formation of S-adenosylmethionine from methionine and ATP. This antibody is specific to MATA2.

Publikationen

Species	Application	Title
human	WB	Mol Cell; mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.; Authors - Elodie Villa ; KD Validated
human	WB,IF	Oxid Med Cell Longev; Oxidative Stress Aggravates Apoptosis of Nucleus Pulposus Cells through m6A Modification of MAT2A Pre-mRNA by METTL16.; Authors - Peng-Bo Chen ; KD Validated
pig	WB	Antioxidants (Basel); M6A RNA Methylation Mediates NOD1/NF-kB Signaling Activation in the Liver of Piglets Challenged with Lipopolysaccharide; Authors - Menghui Xu
mouse	WB	FASEB J; Activating transcription factor 4 regulates angiogenesis under lipid overload via methionine adenosyltransferase 2A-mediated endothelial epigenetic alteration.; Authors - Li Chen ; KD Validated
mouse	WB,IF	FASEB J; Inhibition of MAT2A suppresses osteoclastogenesis and prevents ovariectomy-induced bone loss.; Authors - Honglei Kang ; KD Validated
rat	WB	Environ Toxicol Pharmacol; Ligustrazine modulates renal cysteine biosynthesis in rats exposed to cadmium.; Authors - Wenhua Kuang