Myoglobin Polyklonaler Antikörper

Myoglobin Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 16048-1-AP

Synonyme

MB, MYO, myoglobin

---

• 

  WB analysis using 16048-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16048-1-AP (Myoglobin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 16048-1-AP

  IP result of anti-Myoglobin (IP:16048-1-AP, 4ug; Detection:16048-1-AP 1:1000) with mouse skeletal muscle tissue lysate 2000 ug.

• 

  IHC staining of human heart using 16048-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16048-1-AP (Myoglobin antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 16048-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16048-1-AP (Myoglobin antibody) at dilution of 1:100 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, mouse skeletal muscle, rat heart, rat skeletal muscle
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

16048-1-AP bindet in WB, IHC, IF, IP, ELISA Myoglobin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Myoglobin fusion protein Ag9000
• Vollständiger Name: myoglobin
• Berechnetes Molekulargewicht: 154 aa, 17 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC014547
• Gene symbol: Myoglobin
• Gene ID (NCBI): 4151
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Myoglobin is a cytoplasmic hemoprotein that is expressed primarily in cardiomyocytes and oxidative skeletal muscle fibers, functioning on facilitating oxygen transport and modulating nitric oxide homeostasis within cardiac and skeletal myocytes. Recent studies indicated that myoglobin was also expressed in non-muscle tissues. This antibody well recognized endogenous myoglobin in muscle lysates.

Publikationen

Species	Application	Title
pig	WB	iScience; Dietary protein restriction regulates skeletal muscle fiber metabolic characteristics associated with the FGF21-ERK1/2 pathway; Authors - Shuo Li
mouse	WB	Physiol Rep; Effects of endurance training on the expression of host proteins involved in SARS-CoV-2 cell entry in C57BL/6J mouse.; Authors - Yuki Tamura
mouse	WB	Appl Physiol Nutr Metab; Exercise improves high-fat diet-induced metabolic disorder by promoting HDAC5 degradation through the ubiquitin-proteasome system in skeletal muscle; Authors - Song Huang
human	WB	J Invest Dermatol; N6-methyladenosine modification of lnc-CCKAR-5 regulates autophagy in human umbilical cord mesenchymal stem cells by destabilizing LMNA and inhibits diabetic wound healing; Authors - Jian Wang
human	WB,IF	Inflammation; RNA-seq Based Transcriptome Analysis Reveals Role of Myoglobin in Rheumatoid Arthritis; Authors - Haibin Wang
human	WB,IHC	Int Dent J; Screening Differentially Expressed Proteins in Areca Nut-Related Oral Squamous Cell Carcinoma Using Tandem Mass Tag Proteomics; Authors - Xiaoqun Mai