MBP tag Monoklonaler Antikörper

MBP tag Monoklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

rekombinanten Protein und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(60)

CloneNo.

4C6H4

Rezensionen (1)

Kat-Nr. : 66003-1-Ig

Synonyme

MBP, Maltose Binding Protein

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• Validation Data Gallery
• Proteintech vs. Other Vendors

• 

  WB analysis of Recombinant protein using 66003-1-Ig

  Recombinant protein were subjected to SDS PAGE followed by western blot with 66003-1-Ig (MBP tag antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of Recombinant protein using 66003-1-Ig

  IP result of anti-MBP tag (IP:66003-1-Ig, 5ug; Detection:66003-1-Ig 1:20000) with Recombinant protein protein lysate 800ug.

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  IF Staining of Transfected cells using 66003-1-Ig

  IF result of MBP tag antibody (66003-1-Ig, 1:1,000) with MBP-Tagged protein.Courtesy of Neeraj Tiwari, PhD, Yale School of Medicine, Yale University.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein
• Erfolgreiche IP: Rekombinantes Protein Protein
• Erfolgreiche Detektion in IF/ICC: Transfizierte Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 41 publications below
• IHC: See 1 publications below
• IF: See 6 publications below
• IP: See 6 publications below
• CoIP: See 2 publications below

Produktinformation

66003-1-Ig bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA MBP tag und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MBP tag fusion protein Ag0942
• Vollständiger Name: MBP tag
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein.

Publikationen

Species	Application	Title
	WB,CoIP	Nat Struct Mol Biol; CRISPR-Cas9-based functional interrogation of unconventional translatome reveals human cancer dependency on cryptic non-canonical open reading frames; Authors - Caishang Zheng
	WB	Nat Commun; Structural insights into pathogenic mechanism of hypohidrotic ectodermal dysplasia caused by ectodysplasin A variants; Authors - Kang Yu
	WB	Nat Commun; UV-B irradiation-activated E3 ligase GmILPA1 modulates gibberellin catabolism to increase plant height in soybean; Authors - Jiaqi Sun
	WB	Nat Commun; Cryo-TEM structure of β-glucocerebrosidase in complex with its transporter LIMP-2; Authors - Jan Philipp Dobert
		Mol Cell; Amelioration of hepatic steatosis by dietary essential amino acid-induced ubiquitination.; Authors - Yansong Zhang
		Nucleic Acids Res; The C-terminal 4CXXC-type zinc finger domain of CDCA7 recognizes hemimethylated DNA and modulates activities of chromatin remodeling enzyme HELLS; Authors - Akeo Shinkai

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Daniel (Verified Customer) (08-07-2025)

Antibody successfully detected 50 ng recombinant MBP protein. The signal was very strong!

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: recombinant protein